sp. nov., isolated from the rhizosphere of crop plants.

Two bacterial strains, FP1935 and FP1962, were isolated from the rhizosphere soil of cucumber and Chieh-qua plants, respectively, in Jilin Province, PR China. These strains were Gram-stain-negative, aerobic, rod-shaped and motile with one or two polar flagella. Analysis of the 16S rRNA gene sequences revealed that they represented members of the genus , with the highest similarity to A3 (99.45 %), JAJ28 (99.45 %), NBRC 103147 (99.38 %), P50 (99.27 %) and CFBP 3225 (99.18 %). The DNA G+C contents of FP1935 and FP1962 were 58.99 mol% and 58.98 mol%, respectively. The results of genome-based analyses indicated that these strains were distinct from other species in the genus , as the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were below the recommended thresholds of 95 % (ANI) and 70 % (dDDH) for prokaryotic species delineation, with no values exceeding 94.1 and 55.8 %, respectively, compared with any other related species. The results of phenotypic and chemotaxonomic tests confirmed their differentiation from their closest relatives. The fatty acid profiles of both strains mainly consisted of summed feature 3 (Cω6 and/or Cω7), summed feature 8 (Cω7 and/or Cω6), C and C. The predominant respiratory quinone was Q-9. Polar lipids include phosphatidylethanolamine, unidentified aminophospholipids, unidentified lipids and an unidentified phospholipid. On the basis of these phenotypic and genotypic results, we propose the name sp. nov. for these novel strains. The type strain is FP1935 (=ACCC 62445=JCM 35690).