The objective of this paper is to explore the function of the AOL-s00215g415 (Aog415) gene, which encodes for the synthesis of siderophore in the nematode trapping fungal model strain A. oligospora, in order to understand the relationship between siderophore biosynthesis and nematode trapping activity. After a through sequence analysis, it was determined that Aog415 is a siderophore-synthesizing NRPS. The product of this gene was then identified to be the hydroxamate siderophore desferriferrichrome, using mass spectrometry analysis. When compared to the WT strains, the Aog415 knockout strain exhibited a 60% decrease in siderophore content in fermentation broth. Additionally, the number of predatory rings of decreased by 23.21%, while the spore yield increased by 37.34%. The deletion of Aog415 did not affect the growth of A. oligospora in diverse nutrient medium. Lipid metabolism-related pathways were the primary targets of Aog415 disruption as revealed by the metabolomic analysis. In comparison to the WT, a significant reduction in the levels of glycerophospholipids, and glycolipids was observed in the mutation. The metabolic alteration in fatty acyls and amino acid-like molecules were significantly disrupted. The knockout of Aog415 impaired the biosynthesis of the hydroxamate siderophore desferriferrichrome, remodeled the flow of fatty acid in A. oligospora, and mainly reprogrammed the membrane lipid metabolism in cells. Desferriferrichrome, a hydroxamate siderophore affects the growth, metabolism and nematode trapping ability of A. oligospora by regulating iron intake and cell membrane homeostasis. Our study uncovered the significant contribution of siderophores to the growth and nematode trapping ability and constructed the relationship among siderophores biosynthesis, lipid metabolism and nematode trapping activity of A. oligospora, which provides a new insight for the development of nematode biocontrol agents based on nematode trapping fungi.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/s11274-023-03840-9 | DOI Listing |
Animals (Basel)
November 2024
Wildlife Ecology and Health Group (WE&H), Departament de Ciència Animal, Escola Tècnica Superior d'Enginyeria Agroalimentària i Forestal i de Veterinària (ETSEAFiV), Universitat de Lleida (UdL), 25199 Lleida, Spain.
The prevalence of respiratory nematodes in domestic animals has increased in Europe in recent decades. is the most common parasitic nematode of the respiratory tract in felids, and an increase in its prevalence has been suggested in different European countries including Spain, with values ranging from 0% to 30%. The Baermann technique was used to detect metastrongyloid larvae in 93 faecal samples from cats living in colonies (n = 29), cats living in shelters (n = 30), and owned cats (n = 34).
View Article and Find Full Text PDFParasit Vectors
November 2024
Department for Bacteriology and Parasitology, Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia.
Microorganisms
October 2024
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650500, China.
Curr Opin Plant Biol
December 2024
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA. Electronic address:
Parasit Vectors
November 2024
Department of Zoology, Osun State University, Osogbo, Nigeria.
Background: Entomological data for onchocerciasis surveillance relies on sampling black flies through human landing collectors in the field and laboratory testing of the flies for infection using pooled screening O-150 PCR-ELISA assay. Both techniques require improvements. This study aimed to optimize the Esperanza Window Trap (EWT) for black fly collection.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!