Acidified manure and nitrogen-enriched biochar showed short-term agronomic benefits on cotton-wheat cropping systems under alkaline arid field conditions.

Application of organic residues such as farm manure and biochar in various agricultural environments have shown positive effects on soil carbon sequestration. However, there is a lack of consensus regarding the agronomical benefits of a single and small dose of biochar and farm manure in arid alkaline soils. Therefore, a field experiment with the given treatments (1) control (no amendment), (2) acidified manure (AM) at 300 kg ha, (3) nitrogen (N) enriched biochar (NeB) at 3 Mg ha, and (4) an equal combination of AM + NeB (150 kg ha AM + 1.5 Mg ha NeB)) was conducted in a typical cotton-wheat cropping system. A parallel laboratory incubation study with the same amendments was carried out to account for soil carbon dioxide emission (CO). The N enrichment of biochar and its co-application with acidified manure increased soil mineral N (NO and NH) in the topsoil (0-15 cm), and increased total N uptake (25.92% to 69.91%) in cotton over control, thus reducing N losses and increased uptake over control. Compared to the control, co-application of AM + NeB significantly improved soil N and P bioavailability, leading to increased plant biomass N, P, and K (32%, 40%, 6%, respectively) uptake over control. The plant's physiological and growth improvements [chlorophyll (+ 28.2%), height (+ 47%), leaf area (+ 17%), number of bolls (+ 7%), and average boll weight (+ 8%)] increased the agronomic yield in the first-season crop cotton by 25%. However, no positive response was observed in the second season wheat crop. This field study improved our understanding that co-application of acidified manure and N-enriched biochar in small dose can be a strategy to achieve short-term agronomic benefits and carbon sequestration in the long run.