methyl ganoderate E extends lifespan and modulates aging-related indicators in .

Methyl Ganoderate E (MGE) is a triterpenoid derived from (Reishi), an edible mushroom, commonly processed into food forms such as soups, drinks, culinary dishes, and supplements. MGE has been shown to inhibit 3T3-L1 murine adipocyte differentiation when combined with other triterpenes. However, the specific effect of MGE on biological processes remains unknown. In this study, we present the first evidence of MGE's anti-aging effect in . Through our screening process using the UPR regulation ability, we evaluated a library of 74 pure compounds isolated from , and MGE exhibited the most promising results. Subsequent experiments demonstrated that MGE extended the lifespan by 26% at 10 μg ml through , , and -dependent pathways. MGE also enhanced resistance to various molecular stressors, improved healthspan, increased fertility, and reduced the aggregation of alpha-synuclein and amyloid-beta. Transcriptome data revealed that MGE promoted processes associated with proteolysis and neural activity, while not promoting cell death processes. Collectively, our findings suggest that MGE could be considered as a potential anti-aging intervention, adding to the growing list of such interventions.