Genome-wide identification of the gene family in L. and expression analysis under abiotic and biotic stress.

Background: O-methyltransferase (OMT)-mediated O-methylation is a frequent modification that occurs during natural product biosynthesis, and it increases the diversity and stability of secondary metabolites. However, detailed genome-wide identification and expression analyses of gene family members have not been performed in melons. In this study, we aimed to perform the genome-wide identification of gene family members in melon to identify and clarify their actions during stress.

Methods: Genome-wide identification of gene family members was performed using data from the melon genome database. The OMT genes ( were then compared with the genes from two representative monocotyledons and three representative dicotyledons. The basic information, -regulatory elements in the promoter, predicted 3-D-structures, and GO enrichment results of the 21 were analyzed.

Results: In our study, 21 (named ) were obtained by analyzing the melon genome. These genes were located on six chromosomes and divided into three groups composed of nine, six, and six based on phylogenetic analysis. Gene structure and motif descriptions were similar within the same classes. Each gene contains at least one -acting element associated with hormone transport regulation. Analysis of -acting elements illustrated the potential role of in developmental regulation and adaptations to various abiotic and biotic stresses. The RNA-seq and quantitative real-time PCR (qRT-PCR) results indicated that NaCl stress significantly induced /// and chilling and high temperature and humidity (HTH) stresses significantly upregulated /. Furthermore, the expression pattern of may be associated with f. sp. race 1.2 (FOM1.2) and powdery mildew resistance. Our study tentatively explored the biological functions of genes in various stress regulation pathways and provided a conceptual basis for further detailed studies of the molecular mechanisms.