AI Article Synopsis
- A new high performance liquid chromatography with UV detection (HPLC-UV) method was developed to measure total vitamin D and pre-vitamin D in UV-exposed mushrooms effectively.
- The method includes a two-step solid phase extraction (SPE) to filter out unwanted chromatographic interferences, allowing for accurate quantification of various forms of vitamin D and related compounds.
- Results showed that the vitamin D levels in six types of UV-exposed mushrooms were comparable to those measured by a more complex method (LC-MS), with low limits of quantification, and streamlined sample preparation without the need for drying or large solvents.
Article Abstract
A robust method for quantitation of total vitamin D and D in mushrooms by high performance liquid chromatography with UV detection (HPLC-UV) was developed to analyze mushrooms exposed to UV light. A two-step solid phase extraction (SPE) (silica, carbon black) removed chromatographic interferences typically resolved only with mass spectrometric detection (LC-MS) and allowed quantitation of all vitamin D and pre-D analytes. The vitamin and pre-vitamin forms of D, D and D (internal standard), as well as other photoisomers and sterols were resolved. Results for six types of UV-exposed mushrooms were comparable to LC-MS. Screening of ten additional types of UV-exposed mushrooms without the IS confirmed lack of interference with the IS. The limit of quantification (µg/100 g fresh weight) was 0.4 for vitamin D and 0.9 for pre-vitamin D. Mushrooms do not have to be dried, and separatory funnels and large solvent volumes were also eliminated from sample preparation.
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| http://dx.doi.org/10.1016/j.foodchem.2023.138091 | DOI Listing |
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Article Synopsis
- A new high performance liquid chromatography with UV detection (HPLC-UV) method was developed to measure total vitamin D and pre-vitamin D in UV-exposed mushrooms effectively.
- The method includes a two-step solid phase extraction (SPE) to filter out unwanted chromatographic interferences, allowing for accurate quantification of various forms of vitamin D and related compounds.
- Results showed that the vitamin D levels in six types of UV-exposed mushrooms were comparable to those measured by a more complex method (LC-MS), with low limits of quantification, and streamlined sample preparation without the need for drying or large solvents.
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