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| http://dx.doi.org/10.1111/pbi.14256 | DOI Listing |
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Fusion of a rice endogenous -methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants.
aBIOTECH
June 2024
Institute of Crop Sciences (ICS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081 China.
Unlabelled: Engineering of a new type of plant base editor for simultaneous adenine transition and transversion within the editing window will greatly expand the scope and potential of base editing in directed evolution and crop improvement. Here, we isolated a rice endogenous hypoxanthine excision protein, N-methylpurine DNA glycosylase (OsMPG), and engineered two plant A-to-K (K = G or T) base editors, rAKBE01 and rAKBE02, for simultaneous adenine transition and transversion base editing in rice by fusing OsMPG or its mutant mOsMPG to a plant adenine transition base editor, ABE8e. We further coupled either OsMPG or mOsMPG with a transactivation factor VP64 to generate rAKBE03 and rAKBE04, respectively.
View Article and Find Full Text PDFMixed insect pest populations of Diaspididae species under control of oligonucleotide insecticides: 3'-end nucleotide matters.
Pestic Biochem Physiol
March 2024
Department of Molecular Genetics and Biotechnologies, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Simferopol 295007, Crimea, Ukraine; Laboratory of Entomology and Phytopathology, Dendrology and Landscape Architecture, Nikita Botanical Gardens-National Scientific Centre of the Russian Academy of Sciences, Yalta 298648, Crimea, Ukraine.
Diaspididae are one of the most serious small herbivorous insects with piercing-sucking mouth parts and are major economic pests as they attack and destroy perennial ornamentals and food crops. Chemical control is the primary management approach for armored scale infestation. However, chemical insecticides do not possess selectivity in action and not always effective enough for the control of armored scale insects.
View Article and Find Full Text PDFDevelopment of a high-resolution melt-based assay to rapidly detect the azole-resistant isolates.
Curr Med Mycol
September 2023
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Article Synopsis
- This study focuses on detecting the Y132F mutation in a multidrug-resistant yeast that poses a pandemic threat due to its rapid spread and azole resistance.
- Five isolates from Iran and three control isolates were tested to analyze antifungal susceptibility and develop a high-resolution melt (HRM) assay for mutation detection.
- The HRM method successfully identified the Y132F mutation in one resistant isolate, demonstrating a quick and effective approach to monitor azole resistance in this yeast.
Efficient and heritable A-to-K base editing in rice and tomato.
Hortic Res
January 2024
Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies (Hainan), Institute of Crop Sciences and National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, Hainan 572024, China.
Cytosine and adenosine base editors (CBE and ABE) have been widely used in plants, greatly accelerating gene function research and crop breeding. Current base editors can achieve efficient A-to-G and C-to-T/G/A editing. However, efficient and heritable A-to-Y (A-to-T/C) editing remains to be developed in plants.
View Article and Find Full Text PDFA mutation in CsDWF7 gene encoding a delta7 sterol C-5(6) desaturase leads to the phenotype of super compact in cucumber (Cucumis sativus L.).
Theor Appl Genet
January 2024
College of Life Science, Northwest A&F University, Yangling, 712100, Shaanxi, China.
A novel super compact mutant, scp-3, was identified using map-based cloning in cucumber. The CsDWF7 gene encoding a delta7 sterol C-5(6) desaturase was the candidate gene of scp-3. Mining dwarf genes is important in understanding stem growth in crops.
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