AI Article Synopsis
- Rapid and efficient infection control is essential during outbreaks of carbapenem-resistant Enterobacterales, with FTIR spectroscopy emerging as a promising alternative to the costly and complex WGS.
- A study investigated an outbreak of OXA-48-producing E. coli by using FTIR to determine the relatedness of isolates in real-time and confirmed findings with WGS.
- Results showed that FTIR successfully identified the outbreak cluster's genetic similarities and differences among isolates, suggesting its value as a quick, reliable tool for real-time outbreak investigations.
Article Abstract
Background: Efficient infection control during carbapenem-resistant Enterobacterales outbreaks demands rapid and simple techniques for outbreak investigations. WGS, the current gold standard for outbreak identification, is expensive, time-consuming and requires a high level of expertise. Fourier-transform infrared (FTIR) spectroscopy (IR Biotyper) is a rapid typing method based on infrared radiation applied to samples, which provides a highly specific absorption spectrum.
Objectives: To investigate an outbreak of OXA-48-producing Escherichia coli in real-time using FTIR and subsequently compare the results with WGS.
Methods: Twenty-one isolates were collected during a nosocomial outbreak, and identification and antibiotic susceptibilities were confirmed by VITEK®2. FTIR was conducted for all isolates, and nine representative isolates were sequenced.
Results: FTIR was able to correctly determine the clonal relatedness of the isolates and to identify the outbreak cluster, as confirmed by WGS. By WGS, isolates in the main FTIR cluster belonged to the same MLST type and core-genome MLST type, and they harboured similar plasmids and resistance genes, whereas the singletons external to the FTIR cluster had different genetic content.
Conclusions: FTIR can operate as a rapid, efficient and reliable first-line tool for outbreak investigations during a real-time ongoing E. coli outbreak, which can contribute to limiting the spread of pathogens.
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| http://dx.doi.org/10.1093/jac/dkad387 | DOI Listing |
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