Reprod Biomed Online
Center for Reproductive Medicine, Department of Gynaecology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, China. Electronic address:
Published: February 2024
Research Question: Can rat endometrium be successfully procured and transplanted, and can a similar method be used to procure human endometrium?
Design: Rat endometrium was procured using an endometrium stripping method and transplanted into female Sprague-Dawley rats. Macroscopic and histological changes, endometrial receptivity-related protein concentrations and fertility were assessed. Additionally, a preliminary experiment was conducted to procure human endometrium using a similar method.
Results: Endometrium was successfully procured from both rats and humans, which contained intact endometrium and parts of the adjacent inner annulus myometrium. Endometrium auto-transplantation was conducted in rats and the procedure lasted a total of 41.3 ± 5.7 min with a mean blood loss of 0.09 ± 0.04 g. The transplanted endometrium survived well, but a fibrotic zone formed between the transplant and recipient tissue. Compared with sham rats, those with endometrium transplantation had similar endometrial thickness and endometrial gland numbers but reduced vascular density at 8 weeks after surgery. Endometrium transplantation also retained expression of the endometrial receptivity-related proteins leukaemia inhibitory factor and vascular endothelial growth factor. In contrast to non-pregnancy in the stripped horn, a mean of 5.0 ± 2.7 fetuses developed in the transplanted horn, and full-term live fetuses were conceived in the horns with transplanted endometrium.
Conclusions: Endometrium procurement by stripping method can obtain an intact and functional endometrium, and endometrium transplantation can reconstruct the uterine cavity and restore fertility in rats.
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http://dx.doi.org/10.1016/j.rbmo.2023.103370 | DOI Listing |
Study Question: Does endometriosis affect the mental health of women using oral contraceptives?
Summary Answer: Among oral contraceptive users, women with endometriosis have a higher risk of depression compared to those without endometriosis, although the absolute risk increase is small.
What Is Known Already: Previous studies have suggested a potential link between endometriosis and mental health issues, but the impact of endometriosis on depression among oral contraceptive users remains unclear.
Study Design, Size, Duration: A secondary pooled cohort study utilizing data from two longitudinal patient-centric studies (INAS-VIPOS and PRO-E2) was conducted across 11 European countries, Colombia and Australia.
Reprod Biol
January 2025
Department of Obstetrics and Gynecology, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan.
Endometriosis is an estrogen-dependent benign disease characterized by growth of the endometrial tissue outside the uterine wall. Several reports suggest the possibility of the pathogenesis and recurrence of endometriosis being related to functions of stem/progenitor cells of the endometrium. The drawback of the widely used method of using Hoechst 33342, a fluorescent dye, to collect stem cell-like populations, is the requirement of an ultraviolet (UV) excitation source not commonly provided on standard flow cytometers.
View Article and Find Full Text PDFPak J Pharm Sci
January 2025
Department of Food Science and Nutrition, Alkhurmah University College, Taif University, Taif, Saudi Arabia.
The purpose of the current study was to investigate the potential ameliorating murine reproductive effects of herbal tea extracts against bisphenol A-induced (BPA) cytotoxicity. A comparative study was applied among red, green and blue teas in mice groups. Samples were coded as RTE, GTE and BTE groups, respectively.
View Article and Find Full Text PDFRes Vet Sci
December 2024
Animal and Veterinary Research Center (CECAV), University of Trás-os-Montes e Alto Douro, Quinta de Prados, 500-801 Vila Real, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Portugal. Electronic address:
The Burro de Miranda is an endangered donkey breed. The dynamics of the immune system of the donkey's reproductive tract are essential to manage the fertility of these animals for the improvement and survival of the species. In mares, high numbers of immune cells infiltrating the endometrium promote endometrosis, which is still to be confirmed in jennies.
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