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Time-resolved transcriptomic and proteomic profiling of during NaOH-buffered L-lactic acid production. | LitMetric

AI Article Synopsis

  • * A study utilized transcriptomic and proteomic analyses to examine gene expression changes during L-LA production, finding varying patterns in glycolytic and tricarboxylic acid cycle genes.
  • * Researchers identified gene clusters linked to transcription factors and ABC transporters that increase expression over time, offering insights for metabolic engineering in L-LA fermentation.

Article Abstract

The L-lactic acid (L-LA) fermentation process, based on sodium hydroxide neutralization, demonstrates environmental friendliness during product extraction. However, lactate fermentation is hindered by the pronounced stress effect of sodium lactate on the strain compared with calcium lactate. In this study, we performed time-resolved transcriptomic and proteomic analyses of DSM1 during NaOH-buffered L-LA production. The expression levels of the glycolytic genes demonstrated an initial increase followed by a subsequent decrease, whereas the tricarboxylic acid cycle genes exhibited an initial decrease followed by a subsequent increase throughout the fermentation process. Moreover, we identified clusters of genes consisting of transcription factors and ATP-binding cassette (ABC) transporters that demonstrate a progressive elevation of expression levels throughout the fermentation process, with significant upregulation observed at later stages. This investigation yields valuable insights into the response mechanisms of during NaOH-buffered L-LA fermentation and presents potential targets for metabolic engineering.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10716427PMC
http://dx.doi.org/10.3389/fmicb.2023.1296692DOI Listing

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