AI Article Synopsis
- Bone marrow mesenchymal stem cells (BMMSCs) can differentiate into fat cells, and this study investigates how the compound Icariin (ICA) affects this process, specifically promoting brown fat cell development.
- Researchers used various assays to measure the expression of specific markers and proteins related to fat differentiation and found that ICA boosts the levels of these markers in BMMSCs.
- The study concluded that ICA triggers the differentiation of BMMSCs into brown fat cells by inhibiting PDE5A, suggesting its potential as a treatment for obesity-related conditions.
Article Abstract
Background: Bone marrow mesenchymal stem cells (BMMSCs) possess the ability of adipogenic differentiation. Icariin (ICA) is a prenylated flavonol glycoside with diverse pharmacological activities and has been reported to promote osteogenic differentiation of BMMSCs. Nevertheless, the effects of ICA on BMMSC adipogenic differentiation into brown fat cells are still unclear. This study aimed to explore the effects and mechanistic basis of ICA on the differentiation of BMMSCs into brown fat cells.
Methods: Oil Red-O staining assay was applied to detect the adipogenic differentiation of BMMSCs after induction. RT-qPCR and Western blot were conducted to detect the expression of lipogenic markers PPARγ and FABP4 as well as the brown fat biomarkers BMP7, PGC-1α, and UCP1 in BMMSCs. Moreover, phosphodiesterase-5A (PDE5A) expression in BMMSCs treated with ICA was measured by RT-qPCR and Western blot.
Results: ICA promoted the adipogenic differentiation of BMMSCs and increased the expression levels of lipogenic markers PPARγ and FABP4 and the brown fat biomarkers BMP7, PGC-1α, and UCP1 during the adipogenic differentiation of BMMSCs. Furthermore, PDE5A was identified as a target of ICA, and its expression was reduced by ICA treatment. Moreover, PDE5A inhibition enhanced BMP7, PGC-1α, and UCP1 levels in BMMSCs. Additionally, overexpression of PDE5A notably reversed the effects of ICA in the differentiation of BMMSCs into brown fat cells.
Conclusion: ICA induces the differentiation of BMMSCs into brown fat cells via PDE5A inhibition, highlighting the therapeutic value of ICA for treating obesity-related diseases.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10716507 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2023.e22487 | DOI Listing |
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