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| http://dx.doi.org/10.3389/fpls.2023.1338577 | DOI Listing |
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Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials.
Front Microbiol
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UPR4301 Centre de Biophysique Moléculaire (CBM), Orléans, France.
We investigated the metabolome of the iron- and sulfur-oxidizing, extremely thermoacidophilic archaeon grown on mineral pyrite (FeS). The extraction of organic materials from these microorganisms is a major challenge because of the tight contact and interaction between cells and mineral materials. Therefore, we applied an improved protocol to break the microbial cells and separate their organic constituents from the mineral surface, to extract lipophilic compounds through liquid-liquid extraction, and performed metabolomics analyses using MALDI-TOF MS and UHPLC-UHR-Q/TOF.
View Article and Find Full Text PDFEditorial: Green and sustainable chemistry editor's pick 2024.
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Green Chemistry Centre of Excellence, University of York, York, United Kingdom.
The carbon footprint and energy consumption of liver transplantation.
Front Transplant
January 2025
Department of Surgical, Medical, Biomolecular Pathology and Intensive Care, University of Pisa, Pisa, Italy.
Background And Aims: There is growing interest in the environmental impact of surgical procedures, yet more information is needed specifically regarding liver transplantation. This study aims to quantify the total greenhouse gas emissions, or carbon footprint, associated with adult whole-size liver transplantation from donors after brain death, including the relevant back-table graft preparation.
Methods: The carbon footprint was calculated retrospectively using a bottom-up approach.
Structures and determinants of soil microbiomes along a steep elevation gradient in Southwest China.
Front Microbiol
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School of Ecology and Environment, Tibet University, Lhasa, China.
Soil microbial communities play a vital role in accelerating nutrient cycling and stabilizing ecosystem functions in forests. However, the diversity of soil microbiome and the mechanisms driving their distribution patterns along elevational gradients in montane areas remain largely unknown. In this study, we investigated the soil microbial diversity along an elevational gradient from 650 m to 3,800 m above sea level in southeast Tibet, China, through DNA metabarcode sequencing of both the bacterial and fungal communities.
View Article and Find Full Text PDFLung resection and new postoperative home oxygen requirement: a systematic review.
J Thorac Dis
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Department of Surgery, University of Alabama Birmingham, Birmingham, AL, USA.
Background: While patients are assessed for their ability to tolerate surgery through physiologic evaluations such as pulmonary function tests, ventilation-perfusion scans, and exercising testing, some patients still require home oxygen therapy after pulmonary resection. It is not well understood what the associated risk factors are, how long patients need supplemental oxygen, and if this requirement is associated with worse long-term outcomes. Given these knowledge gaps, we sought to conduct a systematic review of pulmonary resections and new postoperative home oxygen requirement.
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