Peroxidase-Like Activity of Magnetic Nanoparticles in the Presence of Blood Proteins.

The generation of hydroxyl radicals from hydrogen peroxide in aqueous solutions containing magnetic nanoparticles (MNPs), hemoglobin (Hb), immunoglobulin G (IgG), and human serum albumin (HSA) was determined. The dependence of the rate of formation of the oxidized product of o-phenylenediamine (o-PDA) on the concentration of MNPs in solution, as well as on the concentration of proteins, was obtained. The peroxidase-like activity of MNPs was shown to decrease in the presence of HSA and IgG, while the addition of Hb to the reaction mixture led to its decrease and increase depending on protein concentration. The obtained effects can be used in the engineering of systems based on MNPs for theranostics (in particular, for suppression of tumor growth) and in predicting the ability of particles to catalyze the generation of reactive oxygen species (ROS) in vivo.