AI Article Synopsis
- Stabilized trimers of the HIV-1 envelope glycoprotein Env show promise in generating strong and lasting broadly neutralizing antibody responses, making them potential candidates for anti-HIV vaccines.
- A stable producer of the trimer Env recombinant form CRF63_02A6 was created using CHO-K1 cells, which are commonly used in biotechnology.
- Immunochemical assays confirmed that these synthesized trimers were recognized by both monoclonal antibodies and sera from HIV-positive patients, indicating their effectiveness for future vaccine development and structural studies.
Article Abstract
Stabilized trimers of the HIV-1 envelope glycoprotein Env are capable of inducing a potent and sustained broadly neutralizing antibody response in laboratory animals and therefore are attractive targets for anti-HIV vaccine development. In this work, a stable producer of the trimer Env recombinant form CRF63_02A6 of HIV-1 was derived from the CHO-K1 cell line. Using immunochemical assays, the trimers synthesized in CHO-K1 cells were shown to be recognized by both monoclonal broadly neutralizing antibodies and sera from HIV-positive patients. The resulting trimers of the recombinant form CRF63_02A6 of HIV-1 can be used both for structural studies and as a candidate vaccine immunogen against HIV-1.
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| http://dx.doi.org/10.1007/s10517-023-05978-w | DOI Listing |
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