Nanobody-modified gold nanoparticles were used to explore their ability to achieve selective targeting in vitro and in vivo to distinct cell type(s), based on the specificity of the nanobody that was installed. We developed conjugation methods that exploit click chemistry for octahedral ∼50 nm gold nanoparticles and chiral ∼180 nm gold nanoparticles. We determined that each of these particles could be modified with ∼75 and ∼330 nanobodies, respectively. Particle-bound nanobodies retain their antigen binding capacity. After conjugation of the mouse Class II MHC-specific nanobody VHH7 to chiral gold nanoparticles, selective targeting of Class II MHC-positive cell types was observed in vitro by fluorometric assays and by dark-field microscopy. Upon installation of the positron emission tomography (PET) isotopes Zr or Cu on nanobody-modified gold nanoparticles and retro-orbital injection of the radiolabeled particles, we observed accumulation predominantly in the liver and to a far lesser extent in the spleen, regardless of the size of the gold nanoparticles and the identity of the attached nanobody. We observed a striking difference in the distribution of radioisotope-labeled gold nanoparticles by changing the route of administration to intraperitoneal delivery. Significantly reduced accumulation in the liver and spleen was observed by intraperitoneal injection of nanoparticles. In the case of nanobody-modified gold nanoparticles injected intraperitoneally, prominent and persistent signals from the parathymic lymph nodes were observed in the PET/computed tomography images.
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http://dx.doi.org/10.1021/acsami.3c16829 | DOI Listing |
Mikrochim Acta
January 2025
School of Science, Xihua University, Chengdu, 610039, People's Republic of China.
A dual-mode detection platform utilizing colorimetric and Raman was developed based on the exponential amplification reaction (EXPAR) strategy and a "core-satellite" structure constructed by bimetallic nanozymes to detect chloramphenicol (CAP). Initially, DNA-gated metal-organic frameworks (MOFs) incorporating cascaded amplification were used to be nanocarriers for the colorimetric and Raman reporter molecules (3,3',5,5'-tetramethylbiphenyl; TMB). Subsequently, assembled DNA served as gatekeepers to create a stimulus-responsive DNA-gated MOF (TMB@DNA/MOF).
View Article and Find Full Text PDFBioconjug Chem
January 2025
Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD). CT imaging with contrast agents is commonly used for visualizing the gastrointestinal (GI) tract in UC patients. Contrast agents that provide enhanced imaging performance are highly valuable in this field.
View Article and Find Full Text PDFACS Appl Bio Mater
January 2025
Department of Medical Devices, National Institute of Pharmaceutical Education and Research, Guwahati, Assam 781101, India.
Using a laser-scribed (direct printing) technique, we have fabricated an enzymeless, mediatorless, and paper-interfaced electrochemical device (P-LSG) for uric acid detection on a flexible polyimide sheet. Various paper substrates were investigated, and it was found that Whatman filter paper-1 is promising to obtain the best electrochemical signals at the small volume of electrolyte, i.e.
View Article and Find Full Text PDFMacromol Rapid Commun
January 2025
Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, D-01069, Dresden, Germany.
Gold nanoparticles represent nanosized colloidal entities with high relevance for both basic and applied research. When gold nanoparticles are functionalized with polymer-molecule ligands, hybrid nanoparticles emerge whose interactions with the environment are controlled by the polymer coating layer: Colloidal stability and structure formation on the single particle level as well as at the supracolloidal scale can be enabled and engineered by tailoring the composition and architecture of this polymer coating. These possibilities in controlling structure formation may lead to synergistic and/or emergent functional properties of such hybrid colloidal systems.
View Article and Find Full Text PDFCureus
December 2024
Oral and Maxillofacial Surgery, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Pune, IND.
Introduction Endodontic re-infections primarily occur due to the ingress of bacteria and their toxins through an incomplete seal following obturation. A variety of sealers have been developed to achieve effective integration with the different obturation materials and dentinal tubules. To choose the right endodontic sealer and application for each clinical instance, one must be aware of the attributes of the various sealers commonly used in clinical practice.
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