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| http://dx.doi.org/10.1001/jamasurg.2023.5814 | DOI Listing |
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Advancements in delivery strategies and non-tissue culture regeneration systems for plant genetic transformation.
Adv Biotechnol (Singap)
September 2024
Guangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
Plant genetic transformation is a pivotal and essential step in modifying important agronomic traits using biotechnological tools, which primarily depend on the efficacy of transgene delivery and the plant regeneration system. Over the years, advancements in the development of delivery methods and regeneration systems have contributed to plant engineering and molecular breeding. Recent studies have demonstrated that the efficiency of plant transformation can be improved by simultaneously delivering meristem-developmental regulators, utilizing virus-mediated gene editing, and executing non-sterile in planta manipulations.
View Article and Find Full Text PDFA locally administered single-cycle influenza vaccine expressing a non-fusogenic stabilized hemagglutinin stimulates strong T-cell and neutralizing antibody immunity.
J Virol
January 2025
MRC Translational Immune Discovery Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom.
Unlabelled: Current influenza vaccination approaches protect against specific viral strains, but do not consistently induce broad and long-lasting protection to the diversity of circulating influenza viruses. Single-cycle viruses delivered to the respiratory tract may offer a promising solution as they safely express a diverse array of viral antigens by undergoing just one round of cell infection in their host and stimulate broadly protective resident memory T-cell responses in the lung. We have previously developed a vaccine candidate called S-FLU, which is limited to a single cycle of infection by inactivation of the hemagglutinin signal sequence and induces a broadly cross-reactive T-cell response and antibodies to neuraminidase, but fails to induce neutralizing antibodies to hemagglutinin after intranasal administration.
View Article and Find Full Text PDFRates of Multidrug-Resistant Gram-Negative Bacterial Infections in Hospitalized Non-Immunocompromised Pediatric Patients: A 9-Year Retrospective Study at a Lebanese Tertiary Medical Center.
Infect Drug Resist
January 2025
Center for Infectious Diseases Research (CIDR) and WHO Collaborating Center for Reference and Research on Bacterial Pathogens, American University of Beirut, Beirut, Lebanon.
Introduction: Multidrug resistant (MDR) Gram-negative bacterial infections are considered a major public health threat. The objectives of this study were to describe the epidemiology, potential contributing factors, and antimicrobial resistance patterns associated with infections caused by MDR Gram-negative bacteria (GNB) in non-immunocompromised children and adolescents.
Methods: This was a retrospective observational study conducted at the American University of Beirut Medical Center (AUBMC) from 2009 to 2017.
A bridge over troubled water: reverse osmosis to maintain patient care in a boil water notice.
Infect Control Hosp Epidemiol
January 2025
Department of Internal Medicine, Baystate Medical Center, Springfield, MA, USA.
A citywide boil water notice necessitated an alternative solution for treating contaminated water. We report our experience using portable reverse osmosis machines to treat the municipal water to provide purified water to patient care areas where non-sterile water was needed, preventing interruptions in services like elective surgeries.
View Article and Find Full Text PDFEvaluation of Two Different Treatments for Larch Logs as Substrates to Cultivate in the Forest.
Life (Basel)
December 2024
Jilin Academy of Agricultural Sciences, Northeast Agricultural Research Center of China, Changchun 130033, China.
Larch wood, a prevalent cultivation medium for , has yet to be scrutinized concerning the differential impacts of sterilized and non-sterilized substrates on the growth and development of this fungus. Our present investigation sought to elucidate these effects in a forest-like environment. After larch wood segments were sun-dried, they were divided into two groups; one group was bagged and autoclaved, while the other group was bagged without any treatment.
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