Transcriptional regulatory cascade of and affects low-temperature and drought stress response in .

The development of stress tolerance is regulated via the transcriptional regulatory networks involving regulatory homeostasis mediated by protein-DNA interactions. from was characterized to understand the underlying mechanism of low-temperature and drought stress response in . To better understand the transcription pathway of , we cloned the promoter and screened proteins that could interact with the promoter. Using Yeast one-hybrid, electrophoretic mobility shift, and chromatin immunoprecipitation assays, we found that the LcMYB71 protein specifically bound to the promoter of . The transient transformation and stable transgenic system were used to produce transgenic plants with overexpressed and silenced , elucidating the effect of on low-temperature and drought stress tolerance. positively regulated the proline content and enhanced the scavenging of reactive oxygen species, thus improving tolerance to low-temperature and drought stress. Further studies revealed that and had similar functions and could improve plant low-temperature and drought tolerance. It is necessary to identify the upstream regulators of a specific gene to characterize gene functions and the associated transcriptional pathways.