Regel Improves -Induced Inflammatory Responses in a Mouse Ear Edema Model and Suppresses Pro-Inflammatory Chemokine Production the MAPK and NF-κB Pathways in RAW264.7 Cells.

Background: Acne vulgaris (AV) is a common adolescent skin condition which is mainly caused by overcolonization and subsequent inflammation.

Objective: Our previous studies demonstrated that ethanol extracts of Regel (EMQ) possess significant antimicrobial properties. However, their protective effects and potential mechanisms against AV remain unclear.

Methods: In the present study, the EMQ treatment potential for AV was evaluated in a -induced mouse ear edema model, and the EMQ anti-inflammatory mechanism was evaluated in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells.

Results: The results showed that EMQ alleviated edema formation and inflammatory cell infiltration in an acne mouse model by suppressing inflammatory cytokines interleukin (IL)-6, IL-1β, and tumor necrosis factor α expression. Moreover, EMQ inhibited the phosphorylation of MAP kinases (MAPKs) such as p38, JNK, and ERK, the phosphorylation and degradation of IκB-α and the nuclear translocation of nuclear factor kappa B (NF-κB) p65 in LPS-induced RAW264.7 cells.

Conclusion: These findings suggest the potent anti-inflammatory activity of EMQ is possibly through the regulation of the MAPKs and NF-κB signaling pathways. Inhibition of activity combined with a powerful anti-inflammatory effect of EMQ indicated its potential as a novel therapeutic option for AV.