Identification and Characterization of and Causing Stem Rot of White Clover in China.

White clover ( L.) is an excellent quality forage legume species with superior planting efficiency, which reduces the cost of artificial weeding and nitrogen fertilizer inputs, and has feeding and economic value. However, from June to September 2022, severe stem rot affected the yield and quality of white clover crops in Harbin, Heilongjiang Province, China. The aim of this study was to identify the causative agents of the disease. Overall, (6 isolates) and (10 isolates) were obtained from rotten white clover stems and identified based on morpho-molecular characteristics and phylogenetic analyses. Pathogenicity tests of the isolates revealed that had a higher pathogenicity to white clover than . In addition, all isolates were highly pathogenic to broad bean, fodder soybean, soybean, pak choi, and chickpea, were pathogenic to mint, and did not infect corn, wheat, or cilantro. and isolates were very sensitive to tebuconazole and pyraclostrobin, with EC values of 0.54 to 0.70 μg/ml and 0.42 to 0.62 μg/ml, respectively, efficacies ranging between 93.2 to 94.9% and 90.2 to 95.2% at 600 μg/ml and 450 μg/ml, respectively, and EC values of 1.88 to 13.36 μg/ml and 1.32 to 23.39 μg/ml, respectively. Therefore, intercropping of host and nonhost plants and chemicals can be considered to control stem rot in white clover. These results provide a basis for controlling and in white clover in China.