Interaction Between and , Vital for Inhibition.

It was confirmed that the sterile alpha motif and HD domain 1 () limits human immunodeficiency virus type 1 (HIV-1) replication. In contrast, viral protein x () in HIV-2 and some simian immunodeficiency viruses can counteract this effect. The possible interaction between and was suggested by previous studies; however, there are no data to confirm this interaction. Therefore, this study aimed to study the interaction between two proteins and the properties of protein for the first time using bioinformatic tools. and sequences were obtained from the National Center for Biotechnology Information GenBank. Several software were used to define properties and the interaction between and different isoforms. Our findings indicated the difference in interaction sites among different . However, in all proteins, this region is from amino acids 4 to 90. In addition, two regions (26-31 and 134-139) and two amino acids 425 and 429 in are vital in the possible interaction. In addition, our analysis determined the physicochemical and immunological properties of the . Considering all factors, this study could confirm that interacts with , which could inhibit . Moreover, our findings can pave the way for future studies to express and purify in the laboratory and study this protein .