Profiling the antidiabetic potential of GC-MS compounds identified from the methanolic extract of : experimental and computational insight.

This study examines the nutritional composition, phytochemical profiling, and antioxidant, antidiabetic, and anti-inflammatory potential of a methanolic extract of leaves (MESFL) , , and studies. antioxidant, antidiabetic, and anti-inflammatory activities were examined. In the study, liver tissues were subjected to FeSO-induced oxidative damage and treated with varying concentrations of MESFL. MESFL contains a reasonable amount of nitrogen-free extract, moisture, ash content, crude protein, and fat, with a lesser amount of crude fiber. According to GC-MS analysis, MESFL contains ten compounds, the most abundant of which are 13-octadecenal and Ar-tumerone. In this study, MESFL demonstrated anti-inflammatory activities membrane stabilizing properties, proteinase inhibition, and inhibition of protein denaturation (IC = 72.75 ± 11.06 µg/mL). MESFL also strongly inhibited both α-amylase (IC = 307.02 ± 4.25 µg/mL) and α-glucosidase (IC = 215.51 ± 0.47 µg/mL) activities. Our findings also showed that FeSO-induced tissue damage decreased the levels of GSH, SOD, and CAT activities while increasing the levels of MDA. In contrast, treatment with MESFL helped to restore these parameters to near-normal levels, which signifies that MESFL has great potential to address complications from oxidative stress. Furthermore, the interaction of the GCMS-identified phytochemicals with the active sites of α-amylase and α-glucosidase molecular and ensembled-based docking displayed strong binding affinities of Ar-tumerone and 4-hydroxy-3-methylacetophenone to α-amylase and α-glucosidase, respectively. Taken together, the biological activities of MESFL might be a result of the effects of these secondary metabolites.Communicated by Ramaswamy H. Sarma.