The affinity chromatography of Human crude beta-interferon preparations on Blue Dextran Sepharose columns resulted in isolation of several fractions with different ratio of antiviral to antiproliferative activities. The results of investigation of two of these fractions are described in this report. The first of them was eluted by 1N NaCl in 0.01 M tris buffer at pH 7.8, the second was eluted by 1 M NaCl, 50% methylethylenglycol in 0.01 M tris-HCl buffer at pH 7.8. The first of the fractions possessed presumably antiproliferative and the second presumably antiviral activity. Both fractions induced the increase of 2'5'-oligoadenylatesynthetase activity in cells although the inducing activity of the first fraction was about 6-fold higher than that of the second one as compared with their antiviral activities. The obtained results indicate that purification of interferon preparation for interferons main antiviral activity may lead to the loss of the great part of antiproliferative material.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Mixed-mode separation of antisense oligonucleotides using a single column with complementary anion-exchange and hydrophobic interaction chromatography approaches.
J Chromatogr A
January 2025
Genetics Guided Dementia Discovery (G2D2), Eisai, Inc. 35 Cambridge Park Drive, Suite 200, Cambridge, MA, 02140, USA.
The current study investigates the use of mixed-mode chromatography as a combination of anion-exchange (AEX) and hydrophobic interaction chromatography (HIC) for the analysis and purification of single-stranded antisense oligonucleotides with stereo-controlled phosphorothioate inter- nucleotide linkages. Initially a Scherzo-SS-C18 trimodal stationary phase with reversed-phase/AEX/ cation-exchange (CEX) functionalities is systematically evaluated to reveal the presence of U-shaped retention composed of two retention modes namely AEX and HIC, where the latter was also observed on related trimodal Scherzo SM and SW analogues. For the first time, retention and separation of deprotected oligonucleotides was described on a single mixed-mode column using a combination of AEX and HIC.
View Article and Find Full Text PDFEfficient development of nanobody-based affinity chromatography for AAV8 purification.
Protein Expr Purif
March 2025
Shanghai Novamab Biopharmaceuticals Co., Ltd., Shanghai, China. Electronic address:
Article Synopsis
- Adeno-associated virus serotype 8 (AAV8) is a promising vector for gene therapy, but its purification is difficult due to low abundance and strict purity needs.
- This study developed a nanobody (Nb)-based affinity chromatography resin, identifying Nb9 as the best candidate for effectively purifying AAV8 with high binding affinity and yield.
- The purification method showed excellent specificity and consistency across multiple cycles, suggesting it could be a scalable and cost-effective solution for AAV8 purification and highlighting the potential of Nbs in biotechnology.
Water-Soluble Intracellular Polysaccharides (IPSW-2 to 4) from Mycelia: Fractionation, Structural Elucidation, and Antioxidant Activity.
Foods
November 2024
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Article Synopsis
- The study focuses on extracting and analyzing water-soluble intracellular polysaccharides (IPSW-2 to 4) from a medicinal fungus, assessing their antioxidant properties and structures.
- The water-eluted polysaccharide fractions showed higher content and yield compared to salt elution fractions, leading to further purification through size-exclusion chromatography.
- Antioxidant analysis demonstrated that the polysaccharide fractions had better superoxide anion scavenging abilities than polysaccharides from other sources, indicating potential for functional food development.
Hypoglycemic activity of enzymatically extracted Eucommia ulmoides polysaccharide (EUL-w1) on IR-HepG2 cell via the AMPK/PI3K/Akt signaling pathway.
Int J Biol Macromol
December 2024
School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China. Electronic address:
This study devised optimal conditions to extract Eucommia ulmoides leaf (EUL) polysaccharides using a cellulase and pectinase composite enzyme system based on one-way experiments and response surface methodology. Crude EUL polysaccharides (EULPs) were extracted and purified using a DEAE chromatography column. The polysaccharides EUL-w, EUL1, EUL2, and EUL3 were obtained by elution with water, 0.
View Article and Find Full Text PDFBioprospecting amylase from Samiti Lake, situated in the eastern Himalayas.
Int J Biol Macromol
November 2024
Department of Microbiology, University of North Bengal, District Darjeeling 734013, India. Electronic address:
Enzymes, especially amylases, have been an economic boon to the industrial sector, their bioprospective and biotechnological use is an added advantage. Our primary focus of the study was to isolate the most potent amylase producer and to optimize its production parameters through One Factor At A Time (OFAT), Central Composite Rotatable Design Response Surface Methodology (CCRD RSM) and Artificial Neural Network (ANN). Based on the qualitative and quantitative analysis, SLAB1 was selected as the most potent amylase producer out of the potential isolates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!