Introduction: is a traditional starter for dairy products. The lactose rich in milk is the main carbon source for the growth of . However, the utilization of galactose by is strain-specific, and many genetic factors can affect the sugar utilization phenotype of strains.
Methods: In this study, A25, which is capable of utilizing lactose and galactose, was used as the starting strain to construct lactose permease-deficient mutant Δ. Subsequently, the complement vectors expressing complete lactose permease of and its N-terminal 1-486 amino acid residues were constructed and transformed into Δ, respectively. Meanwhile, complement vectors expressing lactose permease and galactose/proton symporter of Escherichia coli were also constructed.
Results And Discussion: Results showed that Δ lost the ability to utilize lactose and galactose. By measuring the growth of the recombinant strains, it was found that the strain expressing complete lactose permease of recovered the growth ability in lactose and galactose medium, while the strain expressing N-terminal of lactose permease recovered the growth ability only in lactose medium. Furthermore, the transformation of Δ was not successful with the complement vector expressing lactose permease, while the strain expressing galactose/proton symporter could recover its growth ability in the galactose medium. These results suggest that the properties of sugar transporters play an important role in galactose utilization by .
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| http://dx.doi.org/10.3389/fmicb.2023.1267237 | DOI Listing |
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