AI Article Synopsis
- Sequencing whole plant genomes is crucial for both applied and fundamental research, particularly in agriculture, as it helps understand beneficial traits of plants.
- Flax is an important crop valued for its oil and fiber, and its genome sequence can provide valuable genetic information for improving cultivated varieties.
- The authors successfully sequenced the first genome of a specific flax variety using Oxford Nanopore and Illumina technologies, achieving a high-quality assembly that enables future studies on plant evolution, domestication, and genome regulation.
Article Abstract
Sequencing whole plant genomes provides a solid foundation for applied and basic studies. Genome sequences of agricultural plants attract special attention, as they reveal information on the regulation of beneficial plant traits. Flax is a valuable crop cultivated for oil and fiber. Genome sequences of its representatives are rich sources of genetic information for the improvement of cultivated forms of the plant. In our work, we sequenced the first genome of flax with the dehiscence of capsules- convar. (Boenn.) Dumort-on the Oxford Nanopore Technologies (ONT) and Illumina platforms. We obtained 23 Gb of raw ONT data and 89 M of 150 + 150 paired-end Illumina reads and tested different tools for genome assembly and polishing. The genome assembly produced according to the Canu-Racon ×2-medaka-POLCA scheme had optimal contiguity and completeness: assembly length-412.6 Mb, N50-5.2 Mb, L50-28, and complete BUSCO-94.6% (64.0% duplicated, eudicots_odb10). The obtained high-quality genome assembly of convar. provides opportunities for further studies of evolution, domestication, and genome regulation in the section .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10702972 | PMC |
| http://dx.doi.org/10.3389/fgene.2023.1269837 | DOI Listing |
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