Gestational monitoring in buffalo females: detection of by PCR.

This study establishes the serological frequency against on day zero and the presence of DNA surveyed throughout the gestation of females in a bovine buffalo system in the central zone of the state of Veracruz, Mexico. Blood samples were taken from 11 females in 6 different sampling periods and analyzed for antibodies detection on day zero. DNA detection by PCR was performed on all sampling periods. The gestation months of the females were recorded for five trimesters by ultrasonography, as well as births and pregnancy losses. Recorded seropositivity and positivity for agent DNA were 90.9% (95% CI 58.7-9.7) and 36.3% (95% CI 10.9-69.2), respectively, on day zero. DNA was detected between 18.1% (95% CI 2.3-51.7) and 45.4% (95% CI 16.7-76.6) over the five trimesters of observation, with three births and three abortions recorded. The studied water buffalo population had a high presence of antibodies; however, the detection of DNA remained below 47% in the females. The association was only observed in the detection of DNA with pregnant females ( 0.007). Our results support the hypothesis of the resistance of water buffaloes to infection and the onset of clinical signs against infection by . even upon a high possibility of infection and reinfection described in this production system in Mexico.