The present experiment was conducted to study the diversity of fruit flies in Nagpur mandarin orchard from different agro-ecological zones of Vidarbha region of Maharashtra, India. The fruit flies samples were collected from 18 places falling under 9 agro-ecological zone using methyl eugenol traps. Out of the total collected catches of fruit flies from each zones, Bactrocera dorsalis was found to be the most prominent species followed by Bactrocera zonata. The Z. cucurbitae and B. correcta which occupied third and fourth rank in species composition, respectively. The species viz., Zeugodacus duplicatus and Zeugodacus gavisus recorded very less abundance at all locations. Shannons index of diversity was maximum in Morshi followed by Katol. Simpson index was more at Tiwasa and Achalpur locations which indicated minimum diversity in the population of fruit flies. Higher diversity of fruit flies were recorded in Morshi and Katol followed by Amravati.
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