Genome-Wide Identification and Comprehensive Analysis of the Gene Family in Soybean ().

The filamentation temperature-sensitive H () gene family is critical in regulating plant chloroplast development and photosynthesis. It plays a vital role in plant growth, development, and stress response. Although genes have been identified in a wide range of plants, there is no detailed study of the gene family in soybean (). Here, we identified 34 genes, which could be categorized into eight groups, and genes in the same group had similar structures and conserved protein motifs. We also performed intraspecific and interspecific collinearity analysis and found that the family has large-scale gene duplication and is more closely related to . Cis-acting elements analysis in the promoter region of the genes revealed that most genes contain developmental and stress response elements. Expression patterns based on transcriptome data and real-time reverse transcription quantitative PCR (qRT-PCR) showed that most of the genes were expressed at the highest levels in leaves. Then, GO enrichment analysis indicated that genes might function as a protein hydrolase. In addition, the GmFtsH13 protein was confirmed to be localized in chloroplasts by a transient expression experiment in tobacco. Taken together, the results of this study lay the foundation for the functional determination of genes and help researchers further understand the regulatory network in soybean leaf development.