The membrane-less organelles in cytoplasm that are presented as cytoplasmic foci were successively identified. Although multiple CCCH zinc-finger proteins have been found to be localized in cytoplasmic foci, the relationship between their specific localization and functions still needs further clarification. Here, we report that the heterologous expression of two CCCH zinc-finger protein genes ( and ) in can affect microgametogenesis by involving the formation of cytoplasmic foci. By monitoring the distribution of proteins and observing pollen phenotypes, we found that, when these two proteins were moderately expressed in pollen, they were mainly dispersed in the cytoplasm, and the pollen developed normally. However, high expression induced the assembly of cytoplasmic foci, leading to pollen abortion. These findings suggested that the continuous formation of BcMF30a/BcMF30c-associated cytoplasmic foci due to high expression was the inducement of male sterility. A co-localization analysis further showed that these two proteins can be recruited into two well-studied cytoplasmic foci, processing bodies (PBs), and stress granules (SGs), which were confirmed to function in mRNA metabolism. Together, our data suggested that BcMF30a and BcMF30c play component roles in the assembly of pollen cytoplasmic foci. Combined with our previous study on the homologous gene of in , we concluded that the function of these homologous genes is conserved and that cytoplasmic foci containing may participate in the regulation of gene expression in pollen by regulating mRNA metabolism.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10706035 | PMC |
| http://dx.doi.org/10.3390/ijms242316862 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Walthard Cell Nests/Transitional Cell Metaplasia in Distal Fallopian Tubes and Pelvic Peritoneum Derived From Reserve Cells.
Int J Gynecol Pathol
December 2024
Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine.
Transitional cell metaplasia (TCM) resembling benign urothelium is commonly seen around the distal fallopian tube and/or neighboring mesothelial surface; however, its histogenesis remains largely unknown. We observed the emergence of a cytokeratin (CK) 17-positive reserve cell layer in early TCM foci beneath the tubal epithelium, leading us to hypothesize that TCM could be derived from reserve cells. To elucidate the histogenetic process of TCM, we analyzed the histomorphologic features and immunoprofiles for CK17, CK5/6, p63, GATA-3, estrogen receptor (ER), and androgen receptor (AR) in TCM foci arising in the tubal epithelium (31 foci) and pelvic mesothelium (35 foci).
View Article and Find Full Text PDFDiversity of the Circulating Tumor Markers: Perspectives of a Multimodal Liquid Biopsy.
Biochemistry (Mosc)
November 2024
N. N. Petrov National Medical Research Center of Oncology, St. Petersburg, 197758, Russia.
Article Synopsis
- Liquid biopsy has become an essential tool over the last decade for diagnosing and managing various malignant tumors, offering benefits like early detection and monitoring of treatment response.
- This method typically analyzes tumor-derived components from blood, including circulating tumor DNA (ctDNA), circulating tumor cells (CTCs), and others, but often fails to detect these markers in a significant number of patients.
- The review highlights the potential of a multiparametric approach that tests multiple biomarkers simultaneously, which could enhance sensitivity and provide a more accurate understanding of cancer progression and treatment outcomes.
-6-methyladenosine (m6A) promotes the nuclear retention of mRNAs with intact 5' splice site motifs.
Life Sci Alliance
February 2025
Department of Biochemistry, University of Toronto, Toronto, Canada
In humans, misprocessed mRNAs containing intact 5' Splice Site (5'SS) motifs are nuclear retained and targeted for decay by ZFC3H1, a component of the Poly(A) Exosome Targeting complex, and U1-70K, a component of the U1 snRNP. In , the ZFC3H1 homolog, Red1, binds to the YTH domain-containing protein Mmi1 and targets certain RNA transcripts to nuclear foci for nuclear retention and decay. Here we show that YTHDC1 and YTHDC2, two YTH domain-containing proteins that bind to -6-methyladenosine (m6A) modified RNAs, interact with ZFC3H1 and U1-70K, and are required for the nuclear retention of mRNAs with intact 5'SS motifs.
View Article and Find Full Text PDFThe RNA-dependent association of phosphatidylinositol 4,5-bisphosphate with intrinsically disordered proteins contribute to nuclear compartmentalization.
PLoS Genet
December 2024
Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
Article Synopsis
- RNA is essential for forming nuclear compartments, with phosphatidylinositol 4,5-bisphosphate (PIP2) playing a key role in RNA transcription and localization in these compartments.
- The study identified the RNA-dependent PIP2-associated (RDPA) nuclear proteome, revealing that proteins in this group often have intrinsically disordered regions that can bind to PIP2 and possess modification sites.
- Highlighting the relationship between PIP2 and RNA, the research found that the protein BRD4 interacts with PIP2 in an RNA-dependent manner, suggesting that PIP2 is crucial for organizing nuclear processes necessary for gene expression.
Trypanosoma cruzi RNA-binding protein DRBD3: perinuclear foci formation during benznidazole exposure.
An Acad Bras Cienc
November 2024
Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Avenida Antônio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil.
Benznidazole (BZ) is the trypanocidal compound of choice for Chagas disease, a neglected tropical disease in the Americas. However, this drug often fails to cure the infection. The regulation of gene expression in Trypanosoma cruzi, the causative agent of Chagas disease, is based on post-transcriptional mechanisms.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!