Feeding Techniques of Green Nanoparticles of Silver and Probiotics: Evaluation of Performance, Physiological, and Microbiological Responses of Hatched One-Day-Old Broiler Chicks.

The aim of this study was to investigate the effects of the feeding of green nanoparticles of silver (Nano-Ag), probiotics, and their combination on hatchability, carcass criteria and internal organs, biochemical parameters, and cecal microbial populations in hatched one-day-old chicks. On day 18 of incubation, 250 live embryo eggs were weighed and randomly assigned to one of five treatment groups: a negative control group, a positive control group consisting of chicks injected with 0.2 mL physiological saline, a group consisting of chicks injected with 0.2 mL Nano-Ag, a group consisting of chicks injected with 0.2 mL probiotics ( spp.), and a group consisting of chicks injected with 0.2 mL combination of Nano-Ag and probiotics (1:1). The results showed that the injection of Nano-Ag or probiotics, alone or in combination, had no effect on hatchability, live body weight, or internal organs but improved ( < 0.05) chick carcass yield compared to the control groups. Furthermore, feeding decreased ( < 0.05) serum levels of cholesterol, triglycerides, urea, creatinine, alanine aminotransferase, and aspartate aminotransferase, as well as cecal , but increased spp. when compared to the control groups. Based on these findings, injections of green Nano-Ag and probiotics, either alone or in combination, have the potential to improve chick health and balance the microbial populations in hatched one-day-old chicks.