AI Article Synopsis
- Researchers developed a method to produce D-arabitol, a valuable chemical, from renewable sources using a specially engineered yeast called Zygosaccharomyces rouxii.
- Genetic modifications enhanced the yeast's ability to create D-arabitol, resulting in a 73.61% increase in production compared to the original strain.
- The study also optimized fermentation conditions and successfully purified D-arabitol to a purity of 96.53%, demonstrating a sustainable approach to manufacturing this compound efficiently.
Article Abstract
Biosynthesis of D-arabitol, a high value-added platform chemical, from renewable carbon sources provides a sustainable and eco-friendly alternative to the chemical industry. Here, a robust brewing yeast, Zygosaccharomyces rouxii, capable of naturally producing D-arabitol was rewired through genome sequencing-based metabolic engineering. The recombinant Z. rouxii obtained by reinforcing the native D-xylulose pathway, improving reductive power of the rate-limiting step, and inhibiting the shunt pathway, produced 73.61% higher D-arabitol than the parent strain. Subsequently, optimization of the fermentation medium composition for the engineered strain provided 137.36 g/L D-arabitol, with a productivity of 0.64 g/L/h in a fed-batch experiment. Finally, the downstream separation of D-arabitol from the complex fermentation broth using an ethanol precipitation method provided a purity of 96.53%. This study highlights the importance of D-xylulose pathway modification in D-arabitol biosynthesis, and pave a complete and efficient way for the sustainable manufacturing of this value-added compound from biosynthesis to preparation.
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| http://dx.doi.org/10.1016/j.biortech.2023.130162 | DOI Listing |
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