Genomic insights into the contribution of de novo lipogenesis to intramuscular fat deposition in chicken.

J Adv Res

State Key Laboratory of Animal Biotech Breeding, State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China. Electronic address:

Published: November 2024

AI Article Synopsis

  • The consumption of chicken is rising globally due to its protein-rich and low-fat properties, making understanding intramuscular fat (IMF) important for meat quality.
  • The study identified fatty acid synthase (FASN) as a crucial gene influencing IMF content and revealed that de novo lipogenesis (DNL) plays a significant role in fat deposition in chicken muscle.
  • These insights enhance our knowledge of fat metabolism in poultry and suggest methods to increase the quality of chicken meat.

Article Abstract

Introduction: The proportion of animal based foods in daily diet of consumers is constantly increasing, with chicken being highly favored due to its high protein and low fat characteristics. The consumption of chicken around the world is steadily increasing. Intramuscular fat (IMF) is a key indicator affecting meat quality.

Object: High IMF content can contribute to improve the quality of chicken meat. The regulatory mechanism of IMF deposition in chicken is poorly understood, so its complete elucidation is essential to improve chicken meat quality.

Method: Here, we performed whole genome resequencing on 516 yellow feather chickens and single-cell RNA sequencing on 3 63-day-old female JXY chickens. In addition, transcriptome sequencing techniques were also performed on breast muscle tissue of JXY chickens at different developmental stages. And C isotope tracing technique was applied.

Results: In this study, a large-scale genetic analysis of an IMF-selected population and a control population identified fatty acid synthase (FASN) as a key gene for improving IMF content. Also, contrary to conventional view, de novo lipogenesis (DNL) was deemed to be an important contributor to IMF deposition. As expected, further analyses by isotope tracing and other techniques, confirmed that DNL mainly occurs in myocytes, contributing about 40% of the total fatty acids through the regulation of FASN, using the available FAs as substrates. Additionally, we also identified a relevant causal mutation in the FASN gene with effects on FA composition.

Conclusion: These findings contribute to the understanding of fat metabolism in muscle tissue of poultry, and provide the feasible strategy for the production of high-quality chicken meat.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519054PMC
http://dx.doi.org/10.1016/j.jare.2023.12.003DOI Listing

Publication Analysis

Top Keywords

chicken meat
12
novo lipogenesis
8
intramuscular fat
8
deposition chicken
8
imf content
8
imf deposition
8
jxy chickens
8
muscle tissue
8
isotope tracing
8
chicken
7

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!