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The process of nucleic acid aptamer selection can be quite laborious and fraught with artifacts. In a work published in Nature Biotechnology, Singh et al. describe an approach that should allow more facile aptamer selection.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10753376 | PMC |
http://dx.doi.org/10.1016/j.crmeth.2023.100667 | DOI Listing |
Anal Biochem
March 2025
Department of Pharmaceutics, College of Pharmacy, Jouf University, P.O. Box 2014, Sakaka 72341, Saudi Arabia. Electronic address:
Aptamers are single-strand oligonucleotide molecules having certain structural interactions which allow them to bind to specific targets. Modified nucleotides are added during or after a selection procedure like Systematic Evolution of Ligands by Exponential Enrichment i.e.
View Article and Find Full Text PDFTalanta
March 2025
Department of Thermal Science and Energy Engineering, University of Science and Technology of China, Hefei, 230027, China. Electronic address:
Aptamers are potent alternatives to antibodies in applications including diagnostics and disease treatment. These synthetic molecules are generated from sequences identified through specific targets within an aptamer pool of random sequences, approximately 10 in size, via the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process. Nevertheless, SELEX encompasses repetitive, time/money-consuming and stochastic methodologies.
View Article and Find Full Text PDFNucleic Acids Res
February 2025
Department of Chemistry, University of Konstanz, Konstanz, Germany.
Riboswitch-mediated control of gene expression without the interference of potentially immunogenic proteins is a promising approach for the development of tailor-made tools for biological research and the advancement of gene therapies. However, the current selection of applicable ligands for synthetic riboswitches is limited and strategies have mostly relied on de novo selection of aptamers. Here, we show that the bacterial xanthine I riboswitch aptamer recognizes oxypurinol, the active metabolite of the widely prescribed anti-gout drug allopurinol (Zyloprim®).
View Article and Find Full Text PDFACS Nano
March 2025
Hunan Institute of Advanced Sensing and Information Technology, Hunan Provincial Key Laboratory of Smart Carbon Materials and Advanced Sensing, Xiangtan University, Xiangtan, Hunan 411105, China.
The urgent need for portable, sensitive, and accurate techniques to analyze multiple antibiotics is critical to mitigating the health risks associated with low-dose antibiotics coexposure-induced drug resistance, especially in infants. Emerging field-effect transistor (FET) biosensors are expected to realize the above requirement, but face challenges in terms of sensitivity and selectivity for complex solutions in practical applications. Here, we introduce a small-molecule coating strategy on carbon nanotube (CNT)-FET biosensor arrays to simultaneously block nonspecific adsorption and minimize Debye shielding effects, coupled with aptamer for antibiotics recognition through inkjet printing technology, which significantly improves the selectivity and sensitivity.
View Article and Find Full Text PDFBackground: In recent years, aptamers have emerged as versatile molecular tools with promising applications in various fields, including diagnostics and therapeutics. In livestock reproduction, their application holds promise for improving the sorting and identification of X and Y chromosome-bearing sperm cells, which is essential for increasing productivity in the dairy and beef industries.
Method: This study utilized seven rounds of Cell-SELEX using bovine X and Y sperm cells to isolate specific aptamers that target these cells.
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