Sensitive Immunochromatographic Determination of in Food Products Using Au@Pt Nanozyme.

In this study, we developed a sensitive immunochromatographic analysis (ICA) of the bacterial pathogen contaminating food products and causing foodborne illness. The ICA of was performed using Au@Pt nanozyme as a label ensuring both colorimetric detection and catalytic amplification of the analytical signal due to nanozyme peroxidase-mimic properties. The enhanced ICA enabled the detection of cells with the visual limit of detection (LOD) of 2 × 10 CFU/mL, which outperformed the LOD in the ICA with traditional gold nanoparticles by two orders of magnitude. The assay duration was 15 min. The specificity of the developed assay was tested using cells from various species as well as other foodborne pathogens; it was shown that the test system detected only . The applicability of ICA for the determination of in food was confirmed in several samples of milk with different fat content, as well as chicken meat. For these real samples, simple pretreatment procedures were proposed. Recoveries of in foodstuffs were from 74.8 to 94.5%. Due to rapidity and sensitivity, the proposed test system is a promising tool for the point-of-care control of the contamination of different food products on the whole farm-to-table chain.