The construction of arrayed mutant libraries has advanced the field of bacterial genetics by allowing researchers to more efficiently study the exact function and importance of encoded genes. In this study, we constructed an arrayed clustered regularly interspaced short palindromic repeats interference (CRISPRi) library, known as rrayed CRISRi (SNAP), as a resource to study >250 essential and growth-supporting genes in . SNAP will be made available to the research community, and we anticipate that its distribution will lead to high-quality, high-throughput, and reproducible studies of essential genes.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10783072 | PMC |
| http://dx.doi.org/10.1128/spectrum.03149-23 | DOI Listing |
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