A novel Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, designated pc2-12, was isolated from the rhizosphere soil of the herb collected from arid areas of Tibet. The strain grew most vigorously with 1 % (w/v) NaCl, at pH 7.0 and at 25 °C. According to the results of 16S rRNA gene sequence analysis, pc2-12 was closely related to the members of the genus , with highest levels of sequence similarity to 687B-08 (98.42 %), 701B-08 (98.11 %) and DSM 18017 (97.98 %). The average nucleotide identity values between pc2-12 and 687B-08, 701B-08 and DSM 18017 were 79.71, 79.49 and 79.26 %, respectively. The DNA-DNA hybridisation values between pc2-12 and . 687B-08, 701B-08 and DSM 18017 were 23.30, 23.00 and 22.90 %, respectively. The draft genome sequence of pc2-12 was 4.64 Mb long, with DNA G+C content of 37.0 mol%. The fatty acids contained in the cells of pc2-12 were mainly composed of iso-C, iso-C 3-OH and summed feature 3 (Cω6 and/or Cω7). The main polar lipid was phosphatidylethanolamine. MK-6 was the sole respiratory quinone. On the basis of the results of analysis of all the data described, pc2-12 is considered to represent a novel species of the genus , for which the name sp. nov., is proposed. The type strain is pc2-12 (=GDMCC 1.3256= JCM 35712).
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http://dx.doi.org/10.1099/ijsem.0.006068 | DOI Listing |
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