Background: Kuth is an aromatic plant used for food seasoning food and in Mexican traditional medicine. Among, leaves properties, it has proven antibacterial activity which can be used against opportunistic pathogens like , a foodborne bacteria that is the causal agent of listeriosis, a disease that can be fatal in susceptible individuals. The aim of this work was to investigate the antibacterial activity of Kuth leaf extracts against and to identify its bioactive components.
Material And Methods: leaves were macerated with four solvents of different polarity (-hexane, dichloromethane, ethyl acetate, and methanol). To determine the capacity to inhibit bacterial proliferation , agar diffusion and microdilution methods were used. Next, we determined the minimal bactericidal concentration (MBC). Finally, we determined the ratio of MBC/MIC. Metabolites present in the active methanolic extract from Kuth (LgMeOH) were purified by normal-phase open column chromatography. The structure of the antibacterial metabolite was determined using nuclear magnetic resonance (H, C, COSY, HSQC) and by comparison with known compounds.
Results: The LgMeOH extract was used to purify the compound responsible for the observed antimicrobial activity. This compound was identified as 5,7-dihydroxyflavanone (pinocembrin) by analysis of its spectroscopic data and comparison with those described. The MIC and MBC values obtained for pinocembrin were 0.68 mg/mL, and the ratio MBC/MIC for both LgMeOH and pinocembrin was one, which indicates bactericidal activity.
Conclusion: Kuth leaves and its metabolite pinocembrin can be used to treat listeriosis due the bactericidal activity against .
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| http://dx.doi.org/10.7717/peerj.16522 | DOI Listing |
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