A novel bacterial strain, GSTT-20 was isolated from an infected, prosthetic endovascular graft explanted from a shepherd in London, United Kingdom. This strain was an aerobic, catalase-positive, oxidase-negative, Gram-stain-negative, motile, curved rod. It grew on blood agar, chocolate agar and MacConkey agar incubated at 37 °C in an aerobic environment after 48 h, appearing as yellow, mucoid colonies. Analysis of the complete 16S rRNA gene sequence showed closest similarity to with 99.6 % identity and with 99.5 % identity. Phylogenetic analysis of the 16S rRNA gene sequence and phylogenomic analysis of single nucleotide polymorphisms within 1530 core genes showed GSTT-20 forms a distinct lineage in the genus of the family . DNA-DNA hybridization assays against GSTT-20 were estimated at 32.1 % for and 31.9 % for . Genome similarity based on average nucleotide identity was 87.50 % when comparing GSTT-20 to . Based on these results, the strain represented a novel species for which the name sp. nov. was proposed. The type strain is GSTT-20 (NCTC 14621=CECT 30390).
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http://dx.doi.org/10.1099/ijsem.0.006184 | DOI Listing |
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