Background And Objectives: In the present study, the anti-biofilm activity of GG and Nisin was investigated on biofilm-forming abilities of strains and the expression of the biofilm-associated genes.
Materials And Methods: In this study, the standard strain of GG (ATCC 53103) and Nisin were used to assess their anti-microbial and anti-biofilm effects on (RP62A).
Results: The MIC and MBC analysis showed that Nisin at 256 μg/mL and 512 μg/mL, and GG at 1×10 CFU/mL and 1×10 CFU/mL have anti-microbial activity compared to the negative control respectively. GG bacteria and Nisin inhibited the biofilm formation of based on optical density of at 570 nm (P <0.001). The relative mRNA expression of and genes was significantly reduced compared to the negative control after treating with sub-MIC of Nisin (0.44, 0.25 and 0.6 fold, respectively) (P>0.05). In addition, the relative expression of and genes, but not (P>0.05), was significantly lower than the negative control (0.62 and 0.7 fold, respectively) (P>0.05), after exposure to the sub MIC of GG.
Conclusion: Nisin and GG exhibit potent activity against biofilm-forming abilities of and these agents could be utilized as an anti-biofilm agents against infections.
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http://dx.doi.org/10.18502/ijm.v15i4.13509 | DOI Listing |
AMB Express
January 2025
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 72388, Sakaka, Saudi Arabia.
Coconut oil is eatable oil with many nutritional and cosmetic applications. In this investigation coconut oil was subjected to 0 to 5 L/min of ozone for 3 h and the chemical composition of both crude and ozonized oil was valued via Gas Chromatography-Mass Spectrometry (GC-MS). Some biological tests were done including antibacterial action versus Helicobacter pylori, anti-biofilm activity versus H.
View Article and Find Full Text PDFBiofouling
January 2025
Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora, México.
This study hypothesizes that eugenol, due to its structural properties, can inhibit glucosyltransferase activity, thereby reducing polysaccharide synthesis in Typhimurium biofilms. It was found that eugenol exhibited minimum inhibitory and bactericidal concentrations of 0.6 mg mL and 0.
View Article and Find Full Text PDFJ Indian Soc Periodontol
December 2024
Department of Periodontology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.
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View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, China.
Human dental caries is an intractable biofilm-associated disease caused by the symbiotic cariogenic bacteria, but how to target effectively eliminate cariogenic bacterial and their biofilms without affecting normal bacteria still remains great challenges. To address this issue, we reported Cu,Fe-doped chitosan-based nanozyme (i.e.
View Article and Find Full Text PDFInfect Dis Rep
December 2024
Microbial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli 620024, India.
Indwelling intrauterine contraceptive devices (IUDs) have surfaces that facilitate the attachment of spp., creating a suitable environment for biofilm formation. Due to this, vulvovaginal candidiasis (VVC) is frequently linked to IUD usage, necessitating the prompt removal of these devices for effective treatment.
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