Background And Objectives: In the present study, the anti-biofilm activity of GG and Nisin was investigated on biofilm-forming abilities of strains and the expression of the biofilm-associated genes.

Materials And Methods: In this study, the standard strain of GG (ATCC 53103) and Nisin were used to assess their anti-microbial and anti-biofilm effects on (RP62A).

Results: The MIC and MBC analysis showed that Nisin at 256 μg/mL and 512 μg/mL, and GG at 1×10 CFU/mL and 1×10 CFU/mL have anti-microbial activity compared to the negative control respectively. GG bacteria and Nisin inhibited the biofilm formation of based on optical density of at 570 nm (P <0.001). The relative mRNA expression of and genes was significantly reduced compared to the negative control after treating with sub-MIC of Nisin (0.44, 0.25 and 0.6 fold, respectively) (P>0.05). In addition, the relative expression of and genes, but not (P>0.05), was significantly lower than the negative control (0.62 and 0.7 fold, respectively) (P>0.05), after exposure to the sub MIC of GG.

Conclusion: Nisin and GG exhibit potent activity against biofilm-forming abilities of and these agents could be utilized as an anti-biofilm agents against infections.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692973PMC
http://dx.doi.org/10.18502/ijm.v15i4.13509DOI Listing

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