AI Article Synopsis
- Caenorhabditis elegans serves as an important model for studying how different organs and cells react to external influences, due to its unique tissue structures.
- The study introduces a new single-nucleus RNA sequencing (snRNA-seq) method that addresses challenges faced with single-cell RNA sequencing in this organism.
- The paper provides a detailed protocol for isolating the nuclei of C. elegans, particularly focusing on analyzing gene expression changes following alcohol exposure, with additional information found in the referenced study by Truong et al. (2023).
Article Abstract
Caenorhabditis elegans is a valuable model to study organ, tissue, and cell-type responses to external cues. However, the nematode comprises multiple syncytial tissues with spatial coordinates corresponding to distinct nuclear transcriptomes. Here, we present a single-nucleus RNA sequencing (snRNA-seq) protocol that aims to overcome difficulties encountered with single-cell RNA sequencing in C. elegans. We describe steps for isolating C. elegans nuclei for downstream applications including snRNA-seq applied to the context of alcohol exposure. For complete details on the use and execution of this protocol, please refer to Truong et al. (2023)..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10730361 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102756 | DOI Listing |
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