AI Article Synopsis
- The study focused on enhancing phenol production from glycerol by genetically engineering the yeast Pichia pastoris.
- By adding tyrosine phenol-lyase, researchers achieved initial phenol yields of 59 mg/L, which significantly increased to 1052 mg/L using a specialized strain in fed-batch fermentation.
- To address the toxic effects of high phenol concentrations on yeast growth, a new separation system was developed, resulting in a remarkable 214% increase in phenol production to 3304 mg/L.
Article Abstract
This study aimed to establish a high-level phenol bioproduction system from glycerol through metabolic engineering of the yeast Pichia pastoris (Komagataella phaffii). Introducing tyrosine phenol-lyase to P. pastoris led to a production of 59 mg/L of phenol in flask culture. By employing a strain of P. pastoris that overproduces tyrosine-a precursor to phenol-we achieved a phenol production of 1052 mg/L in glycerol fed-batch fermentation. However, phenol concentrations exceeding 1000 mg/L inhibited P. pastoris growth. A phenol pertraction system utilizing a hollow fiber membrane contactor and tributyrin as the organic solvent was developed to reduce phenol concentration in the culture medium. Integrating this system with glycerol fed-batch fermentation resulted in a 214 % increase in phenol titer (3304 mg/L) compared to glycerol fed-batch fermentation alone. These approaches offer a significant framework for the microbial production of chemicals and materials that are highly toxic to microorganisms.
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| http://dx.doi.org/10.1016/j.biortech.2023.130144 | DOI Listing |
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