Preserving rumen fluid as the inoculum for anaerobic digestion of food waste is necessary when access to animal donors or slaughterhouses is limited. This study aims to compare two preservation methods relative to fresh ruminal inoculum: (1) cryoprotected with 5% dimethyl sulfoxide (DMSO) and stored at -20 °C and (2) frozen at -20 °C, both for 6 months. The fermentation activity of different inoculum was evaluated by rumen-based in vitro anaerobic fermentation tests (volatile fatty acids, biomass digestibility, and gas production). Citrus pomace was used as the substrate during a 96-h fermentation. The maximum volatile fatty acids, methane production, and citrus pomace digestibility from fresh rumen fluid were not significantly different from rumen fluid preserved with DMSO. Metagenome analysis revealed a significant difference in the rumen microbial composition and functions between fresh rumen fluid and frozen inoculum without DMSO. Storage of rumen fluid using -20 °C with DMSO demonstrated the less difference compared with fresh rumen fluid in microbial alpha diversity and taxa composition. The hierarchical clustering tree of CAZymes showed that DMSO cryoprotected fluid was clustered much closer to the fresh rumen fluid, showing more similarity in CAZyme profiles than frozen rumen fluid. The abundance of functional genes associated with carbohydrate metabolism and methane metabolism did not differ between fresh rumen fluid and the DMSO-20 °C, whereas the abundance of key functional genes significantly decreased in frozen rumen fluid. These findings suggest that using rumen liquid preserved using DMSO at -20 °C for 180 days is a feasible alternative to fresh rumen fluid. This would reduce the need for laboratories to maintain animal donors and/or reduce the frequency of collecting rumen fluid from slaughterhouses.
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http://dx.doi.org/10.1016/j.jenvman.2023.119693 | DOI Listing |
J Anim Physiol Anim Nutr (Berl)
January 2025
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
The objective of this study was to examine the impact of black soldier fly larval oil (BSFO) on feed consumption, nutritional digestibility, ruminal characteristics and methane (CH) estimation in Thai-indigenous steers. Four male Thai native steers (Bos indicus) weighing 383 ± 9.0 kg were used in this investigation.
View Article and Find Full Text PDFAnimals (Basel)
December 2024
Jiangxi Province Key Laboratory of Animal Nutrition and Feed, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
This study aimed to investigate the effects of different storage times of the mixed inoculum on in vitro rumen fermentation characteristics, microbial diversity, and community composition. The experiment was divided into five groups, with mixed inoculum composed of fresh rumen fluid and culture medium being stored at 39 °C for 0 h (H0), 12 h (H12), 24 h (H24), 36 h (H36), and 48 h (H48). After 48 h of in vitro fermentation, the fermentation fluid was collected to assess rumen fermentation characteristics and microbial community composition.
View Article and Find Full Text PDFJ Dairy Sci
January 2025
Department of Applied Animal Science & Welfare, Swedish University of Agricultural Sciences (SLU), Box 7024, 753 23 Uppsala, Sweden.
Methane emissions from ruminant digestion contribute significantly to global anthropogenic greenhouse gas emissions. Members of the phylum Rhodophyta (red algae), particularly Asparagopsis sp., have shown promising results in reducing methane emissions in ruminants, due to their high content of halogenated methane analog compounds.
View Article and Find Full Text PDFTrop Anim Health Prod
January 2025
Federal Institute of Education Science and Technology of Piauí (IFPI), Campus of José de Freitas, Rua da Barragem Do Bezerro, S/N, Bezerro, José de Freitas, PI, 64110-000, Brazil.
The Caatinga biome has a wide variety of plants which, despite their irregular distribution, are frequently used to feed animals. This study aimed to evaluate the gas production kinetics of Caatinga plants: Malva (Herissantia crispa), Aroeira (Myracrodruon urundeuva), Marmeleiro (Croton sonderianus), and Bamburral (Hyptis suaveolens). Buffel grass (Cenchrus ciliaris), a cultivated forage, was used as a control.
View Article and Find Full Text PDFAnimals (Basel)
December 2024
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
This study hypothesized that combining oregano essential oil () and yeast cultures (s) would modulate rumen microbiota to promote gastrointestinal homeostasis and function. Twenty-four newborn, healthy, disease-free, crossbred Simmental male calves (birth weight ≥ 35 kg) were assigned to one of four treatments based on birth data. Treatments were as follows: (1) Control (), calves fed calf starter without additives; (2) , calves fed calf starter containing 60 mg/kg body weight () of OEO per day; (3) s, calves fed calf starter containing 45 mg/kg BW of YC per day; and (4) , calves fed calf starter with OEO (60 mg/kg, BW) and YC (45 mg/kg, BW) combination.
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