Differentiating among nonpneumococcal viridans group streptococci (VGS) is challenging in conventional laboratories. Therefore, we aimed to evaluate the performance of the latest Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system in identifying VGS by comparing the results to those of the specific gene sequencing approach. Clinical isolates were initially identified using the BD Phoenix system to identify species. The optochin test was used to distinguish nonpneumococcal VGS from . The species of individual reference strains and clinical isolates were determined by comparing the sequences of the 16S rDNA, , , , or genes with those in the GenBank sequence databases. We evaluated the performance of the Bruker Biotyper MALDI-TOF MS in VGS identification using two different machines with three databases. We collected a total of 103 nonpneumococcal VGS and 29 . blood isolates at a medical center in northern Taiwan. Among these isolates, only seven could not be identified at the species level by the specific gene sequencing approach. We found that none of the nonpneumococcal VGS isolates were misidentified as pneumococci by the latest Biotyper system, and vice versa. However, certain strains, especially those in the mitis and bovis groups, could still not be correctly identified. The latest Bruker Biotyper 4.1 (DB_10833) showed significant improvement in identifying VGS strains. However, a specific gene sequencing test is still needed to precisely differentiate the species of strains in the mitis and bovis groups.
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http://dx.doi.org/10.1128/jcm.01143-23 | DOI Listing |
J Infect Chemother
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Department of Gastroenterology, Nagoya City University East Medical Center, Aichi, Japan.
Vibrio fluvialis is a halophilic, motile, flagellated, gram-negative bacterium commonly associated with acute gastroenteritis. However, extraintestinal infections are rare. We describe an unusual case of V.
View Article and Find Full Text PDFJ Clin Microbiol
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School of Medicine, College of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Unlabelled: BluePoint MoldID can identify 43 fungal species through nucleic acid array hybridization and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can identify 247 filamentous fungi through mass spectrometry. First, 43 standard isolates from the Bioresource Collection and Research Center, Taiwan, and the College of American Pathologists and 41 clinical species isolates confirmed by rDNA-ITS sequencing were analyzed using BluePoint MoldID and Bruker MALDI-TOF MS. BluePoint MoldID accurately identified 79% (34/43) of the standard isolates to the species level but failed to recognize nine isolates absent from its database; additionally, 87.
View Article and Find Full Text PDFEur J Clin Microbiol Infect Dis
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Faculty of Medicine, Department of Medical Microbiology, Dokuz Eylül University, İnciraltı, İzmir, 35340, Turkey.
Antibiotics (Basel)
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Clinical Microbiology Laboratory, University Hospital "St. Marina"-Varna, 9010 Varna, Bulgaria.
J Food Prot
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Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, A.L. 2204E, Ottawa, Ontario K1A 0K9, Canada. Electronic address:
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