Our results demonstrate increased extracellular ammonium release in the endophyte plant growth-promoting bacterium . Strains were constructed in a manner that leaves no antibiotic markers behind, such that these strains contain no transgenes. Levels of ammonium achieved by cultures of modified strains reached concentrations of approximately 18 mM ammonium, while wild-type remained much lower (below 50 µM). These findings demonstrate a strong potential for further improving the biofertilizer potential of this important microbe.
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http://dx.doi.org/10.1128/spectrum.02478-23 | DOI Listing |
World J Microbiol Biotechnol
January 2025
Engineering Research Centre for Waste Oil Recovery Technology and Equipment, Ministry Education, Chongqing Technology and Business University, Chongqing, 400067, China.
In recent years, it has become widely acknowledged that heavy metals are often present in oil-contaminated sites. This study utilized three specific types of microorganisms with different functions to construct a composite bacterial consortium for treating lubricant-Cr(VI) composite pollutants. The selected strains were Lysinbacillus fusiformis and Bacillus tropicus.
View Article and Find Full Text PDFMicroorganisms
December 2024
Division of Biotechnology and Advanced Institute of Environment and Bioscience, Jeonbuk National University, Iksan 54596, Jeonbuk, Republic of Korea.
A Gram-positive, rod-shaped, and obligate anaerobic bacterial strain OS1-26 was isolated from apple orchard soil in Iksan, South Korea. Interestingly, strain OS1-26 was observed to possess the functional genes involved in biological nitrogen fixation (BNF), including , which was actively transcribed during the anaerobic cultivation with excessive production of extracellular NH despite of presence of other fixed N nutrients. The BNF of strain OS1-26 was distinguished from the other well-known diazotrophs, such as and .
View Article and Find Full Text PDFKidney Dis (Basel)
November 2024
Department of Geriatric Urology, Xiangya International Medical Center, Xiangya Hospital, Central South University, Changsha, PR China.
Introduction: This study aims to explore the contribution of neutrophil extracellular traps (NETs) to kidney stones.
Methods: The microarray data from GSE73680 and bioinformatic analysis were applied to identify differentially expressed genes in patients with kidney stones. A rat model of kidney stones was established through ethylene glycol and ammonium chloride administration.
Heliyon
December 2024
Microelement Research Center of Huazhong Agricultural University, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Wuhan, Hubei Province, 430070, China.
Chinese milk vetch (CMV) is widely recognized as the leading leguminous green manure utilized in the rice-green manure rotation system throughout southern China. While bacteria that form symbiotic relationships with CMV are responsible for fixing a significant portion of nitrogen (N) within agroecosystems. diazotrophic organisms play an essential role in the N cycle and enhance the pool of N readily accessible to plants.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Department of Environmental Science and Engineering, Zhejiang Ocean University, No.1 Haida South Road, Zhoushan, 316022, PR China; Zhejiang Provincial Key Laboratory of Petrochemical Environmental Pollution Control, Zhoushan, 316022, PR China. Electronic address:
In order to study the resistance mechanisms of biofilm and granular sludge to various dissolved oxygen (DO) exposures in anaerobic ammonium oxidation (anammox) process, a biofilm - granular sludge anammox reactor was established and operated. Experimental results showed that DO levels of ≤0.41 mg L hardly affected the total nitrogen removal efficiency (TNRE).
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