Enhanced extracellular ammonium release in the plant endophyte through genome editing.

Microbiol Spectr

Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, Minnesota, USA.

Published: January 2024

Our results demonstrate increased extracellular ammonium release in the endophyte plant growth-promoting bacterium . Strains were constructed in a manner that leaves no antibiotic markers behind, such that these strains contain no transgenes. Levels of ammonium achieved by cultures of modified strains reached concentrations of approximately 18 mM ammonium, while wild-type remained much lower (below 50 µM). These findings demonstrate a strong potential for further improving the biofertilizer potential of this important microbe.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10783055PMC
http://dx.doi.org/10.1128/spectrum.02478-23DOI Listing

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