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| http://dx.doi.org/10.1172/JCI173715 | DOI Listing |
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High-frequency home self-collection of capillary blood correlates IFI27 expression kinetics with SARS-CoV-2 viral clearance.
J Clin Invest
December 2023
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, Washington, USA.
RNA self-blood collection enables high-frequency temporal profiling of presymptomatic host immune kinetics to respiratory viral infection: a prospective cohort study.
medRxiv
September 2024
Department of Chemistry, University of Washington; Seattle, WA, U.S.A.
Article Synopsis
- This study investigates the dynamics of early immune responses to acute respiratory infections (ARIs), focusing on how an individual's immune system reacts before and after symptoms appear.
- Researchers used a self-sampling method to collect blood and nasal swabs from participants daily for a week and weekly afterward, analyzing samples to monitor immune gene activity and symptom development.
- A total of 68 participants contributed samples over the study period, with notable findings including that SARS-CoV-2 was detected in various participants, even when they showed no symptoms, offering insights into presymptomatic immune profiles.
Longitudinal home self-collection of capillary blood using RNA correlates interferon and innate viral defense pathways with SARS-CoV-2 viral clearance.
medRxiv
January 2023
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center; Seattle, Washington, U.S.A.
Article Synopsis
- Blood transcriptional profiling is used to evaluate immune responses to infections, but traditional blood collection methods hinder accurate analysis during dynamic infections like COVID-19.
- A new at-home self-collection method allows participants to collect blood and nasal swabs every other day, enabling high-frequency measurement of immune response during acute SARS-CoV-2 infections.
- The study found significant differences in immune responses between healthy and COVID-19+ individuals, revealing distinct patterns based on vaccination status, and demonstrating the effectiveness of at-home sampling in tracking immune kinetics.
Finger stick blood collection for gene expression profiling and storage of tempus blood RNA tubes.
F1000Res
June 2016
Systems Biology Department, Sidra Medical and Research Center, Doha, Qatar.
With this report we aim to make available a standard operating procedure (SOP) developed for RNA stabilization of small blood volumes collected via a finger stick. The anticipation that this procedure may be improved through peer-review and/or readers public comments is another element motivating the publication of this SOP. Procuring blood samples from human subjects can, among other uses, enable assessment of the immune status of an individual subject via the profiling of RNA abundance using technologies such as real time PCR, NanoString, microarrays or RNA-sequencing.
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