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Untargeted metabolomics identifies metabolic dysregulation of sphingolipids associated with aggressive chronic lymphocytic leukaemia and poor survival. | LitMetric

Untargeted metabolomics identifies metabolic dysregulation of sphingolipids associated with aggressive chronic lymphocytic leukaemia and poor survival.

Clin Transl Med

Centre Hospitalier Universitaire de Québec Research Center-Université Laval (CRCHUQc-UL), Faculty of Pharmacy and Centre de Recherche sur le Cancer (CRC-UL), Université Laval, Québec, Canada.

Published: December 2023

AI Article Synopsis

  • The study explores the metabolic characteristics of chronic lymphocytic leukaemia (CLL) cells to identify potential personalized treatment options for patients with aggressive forms of the disease.
  • Researchers employed metabolomics and lipidomics to analyze associations between specific metabolites, particularly glycosyltransferase (UGT2B17), and poor survival outcomes in CLL patients.
  • Findings revealed that certain sphingolipids, like glucosylceramides, serve as prognostic markers linked to treatment-free survival, and targeting their synthesis could enhance the effectiveness of existing CLL therapies.

Article Abstract

Background: Metabolic dependencies of chronic lymphocytic leukaemia (CLL) cells may represent new personalized treatment approaches in patients harbouring unfavourable features.

Methods: Here, we used untargeted metabolomics and lipidomics analyses to isolate metabolomic features associated with aggressive CLL and poor survival outcomes. We initially focused on profiles associated with overexpression of the adverse metabolic marker glycosyltransferase (UGT2B17) associated with poor survival and drug resistance.

Results: Leukaemic B-cell metabolomes indicated a significant perturbation in lipids, predominantly bio-active sphingolipids. Expression of numerous enzyme-encoding genes of sphingolipid biosynthesis pathways was significantly associated with shorter patient survival. Targeted metabolomics further exposed higher circulating levels of glucosylceramides (C16:0 GluCer) in CLL patients relative to healthy donors and an aggressive cancer biology. In multivariate analyses, C16:0 GluCer and sphinganine were independent prognostic markers and were inversely linked to treatment-free survival. These two sphingolipid species function as antagonistic mediators, with sphinganine being pro-apoptotic and GluCer being pro-proliferative, tested in leukemic B-CLL cell models. Blocking GluCer synthesis using ceramide glucosyltransferase inhibitors induced cell death and reduced the proliferative phenotype, which further sensitized a leukaemic B-cell model to the anti-leukaemics fludarabine and ibrutinib in vitro.

Conclusions: Specific sphingolipids may serve as prognostic markers in CLL, and inhibiting enzymatic pathways involved in their biosynthesis has potential as a therapaeutic approach.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689972PMC
http://dx.doi.org/10.1002/ctm2.1442DOI Listing

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