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Cold snapshots of DNA repair: Cryo-EM structures of DNA-PKcs and NHEJ machinery. | LitMetric

Cold snapshots of DNA repair: Cryo-EM structures of DNA-PKcs and NHEJ machinery.

Prog Biophys Mol Biol

Leicester Institute for Structural and Chemical Biology, Department of Molecular and Cell Biology, University of Leicester, Leicester, UK. Electronic address:

Published: January 2024

AI Article Synopsis

Article Abstract

The proteins and protein assemblies involved in DNA repair have been the focus of a multitude of structural studies for the past few decades. Historically, the structures of these protein complexes have been resolved by X-ray crystallography. However, more recently with the advancements in cryo-electron microscopy (cryo-EM) ranging from optimising the methodology for sample preparation to the development of improved electron detectors, the focus has shifted from X-ray crystallography to cryo-EM. This methodological transition has allowed for the structural determination of larger, more complex protein assemblies involved in DNA repair pathways and has subsequently led to a deeper understanding of the mechanisms utilised by these fascinating molecular machines. Here, we review some of the key structural advancements that have been gained in the study of non-homologous end joining (NHEJ) by the use of cryo-EM, with a focus on assemblies composed of DNA-PKcs and Ku70/80 (Ku) and the various methodologies utilised to obtain these structures.

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Source
http://dx.doi.org/10.1016/j.pbiomolbio.2023.11.007DOI Listing

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