AI Article Synopsis
- - The study aimed to assess how effective metagenomic next-generation sequencing (mNGS) is in diagnosing infections related to marine trauma at a hospital in Zhuhai, analyzing data from 14 patients over a 3-year period.
- - Most patients (78.6%) had marine-related injuries, while infections were confirmed through a combination of culture and mNGS methods, with a significant recovery rate following surgical intervention and antibiotic treatment.
- - The findings highlight that mNGS is valuable for diagnosing water-exposed infections and can help identify multiple pathogens, improving treatment guidance for severe infections with unknown causes.
Article Abstract
Objective: To evaluate the diagnostic value of metagenomic next-generation sequencing (mNGS) in () infection.
Methods: A retrospective analysis of patients with infection at the Fifth Affiliated Hospital of Sun Yat-Sen University from January 1, 2020 to April 23, 2023 was conducted. 14 enrolled patients were diagnosed by culture or mNGS. The corresponding medical records were reviewed, and the clinical data analyzed included demographics, epidemiology laboratory findings, physical examination, symptoms at presentation, antibiotic and surgical treatment, and outcome.
Results: In this study, 78.6% (11/14) patients had a history of marine trauma (including fish stab, shrimp stab, crab splints and fish hook wounds), 7.1% (1/14) had eaten seafood, and the remaining 14.3% (2/14) had no definite cause. Isolation of from clinical samples including blood, tissue, fester and secreta. 9 cases were positive for culture, 5 cases were detected synchronously by mNGS and got positive for . 85.7% (12/14) cases accepted surgical treatment, with 1 patient suffering finger amputated. 14 enrolled patients received appropriate antibiotic therapy, and all of them had recovered and discharged. 9 strains isolated in this study were sensitive to most beta-lactam antibiotics, aminoglycosides, quinolones, etc.
Conclusion: infection is a common water-exposed disease in Zhuhai, which requires identification of a number of pathogens. Of severe infections with unknown pathogen, mNGS can be used simultaneously, and the potential to detect multiple pathogens is of great help in guiding treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687401 | PMC |
| http://dx.doi.org/10.3389/fcimb.2023.1210919 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pulmonary mucormycosis in an immunocompetent young female: a case report and literature review.
Front Med (Lausanne)
December 2024
Department of ICU, Daping Hospital, Army Medical University, Chongqing, China.
Mucormycosis is considered a rare but highly lethal fungal infection, often occurring in patients with poorly controlled diabetes or immunosuppression. Pulmonary mucormycosis progresses rapidly and is often associated with pulmonary infarction and hemoptysis. In this case report, we presented a young, immunocompetent female patient with newly diagnosed diabetes who was diagnosed early with infection through metagenomic next-generation sequencing.
View Article and Find Full Text PDFUnveiling the dark side of : a case of fatal pneumonia from a common probiotic.
Front Med (Lausanne)
December 2024
Department of Cardiology, General Hospital of Central Theater Command of the People's Liberation Army, Wuhan, China.
is often considered a complex associated with a healthy plant-based diet that acts as a "probiotic" throughout the body's entire digestive tract from the mouth to the anus. Previous studies have not reported that this "probiotic" colonizing the human body could cause severe pneumonia. This case report describes a 56-year-old healthy female worker with gum pain followed by fever.
View Article and Find Full Text PDFA Rare Q-Fever Infection Diagnosed Using Metagenomic Next-Generation Sequencing in Liver Transplantation Patient: A Case Report and Literature Review.
Infect Drug Resist
January 2025
Department of Organ Transplantation, The Third Medical Center of Chinese PLA General Hospital, Beijing, People's Republic of China.
Q fever is a zoonotic disease caused by the Gram-negative bacterium , typically transmitted through exposure to infected animal secretions. As the clinical signs of Q-fever are largely non-specific in humans, a definitive diagnosis can often be overlooked, particularly when physicians fail to consider on the list of differentials. This case report describes Q-fever in a male patient who had previously undergone orthotopic liver transplantation.
View Article and Find Full Text PDFMetagenomic next-generation sequencing of cerebrospinal fluid: a diagnostic approach for varicella zoster virus-related encephalitis.
Front Cell Infect Microbiol
December 2024
Department of Neurology, Harrison International Peace Hospital, Hengshui, China.
Purpose: Varicella zoster virus-related encephalitis (VZV-RE) is a rare and often misdiagnosed condition caused by an infection with the VZV. It leads to meningitis or encephalitis, with patients frequently experiencing poor prognosis. In this study, we used metagenomic next-generation sequencing (mNGS) to rapidly and accurately detect and identify the VZV pathogen directly from cerebrospinal fluid (CSF) samples, aiming to achieve a definitive diagnosis for encephalitis patients.
View Article and Find Full Text PDFIdentification of Anthrax as the Cause of a Cluster of Unexplained Deaths, Uganda, 2023: The Role of Metagenomic Next-Generation Sequencing and Postmortem Specimens.
Am J Trop Med Hyg
January 2025
MRC/UVRI & LSHTM Uganda Research Unit, Entebbe, Uganda.
Between April and November 2023, 27 unexplained human deaths that presented with swelling of the arms, skin sores with black centers, difficulty in breathing, obstructed swallowing, headaches, and other body aches were reported in Kyotera District, Uganda by the Public Health Emergency Operations Center. Subsequently, the death of cattle on farms and the consumption of carcass meat by some residents were also reported. Field response teams collected clinical/epidemiological data and autopsy samples to determine the cause of deaths.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!