An antibiotic produced by CFBP2392 with antifungal activity against .

CFBP2392 has been recognized as a potential biocontrol agent due to its ability to suppress damping-off and root rot disease. This isolate has antibacterial activity as many other strains from the complex. In this work, the antibacterial and antifungal activity of the strain were explored. Dual culture assays evidenced the antifungal activity of the strain against different phytopathogens: sp., , , and . Purification of an antifungal fraction was performed by preparative HPLC from the chemical extraction of growth media. The fraction showed altered growth and ultrastructure. Transmission electron microscopy revealed the purified compound hypertrophied mitochondria, membranous vesicles, and a higher number of vacuoles in cytoplasm. In addition, co-cultivation of CFBP2392 with resulted in an enlarged and deformed cell wall. To gain genomic insights on this inhibition, the complete genome of CFBP2392 was obtained with Oxford Nanopore technology. Different biosynthetic gene clusters (BGCs) involved in specialized metabolites production including a lokisin-like and a koreenceine-like cluster were identified. In accordance with the putative BGCs identified, sequence phylogeny analysis of the MacB transporter in the lokisin-like cluster further supports the similarity with other transporters from the amphisin family. Our results give insights into the cellular effects of the purified microbial metabolite in ultrastructure and provide a genomic background to further explore the specialized metabolite potential.