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Development of an Electrochemical, Aptamer-Based Sensor for Dynamic Detection of Neuropeptide Y. | LitMetric

Development of an Electrochemical, Aptamer-Based Sensor for Dynamic Detection of Neuropeptide Y.

ACS Sens

Department of Chemistry, University of Cincinnati, 312 College Dr. 404 Crosley Tower, Cincinnati, Ohio 45221-0172, United States.

Published: December 2023

AI Article Synopsis

Article Abstract

The ability to monitor dynamic changes in neuropeptide Y (NPY) levels in complex environments can have an impact on many fields, including neuroscience and immunology. Here, we describe the development of an electrochemical, aptamer-based (E-AB) sensor for the dynamic (reversible) measurement of physiologically relevant (nanomolar) concentrations of neuropeptide Y. The E-AB sensors are fabricated using a previously described 80 nucleotide aptamer reported to specifically bind NPY with a binding affinity = 0.3 ± 0.2 uM. We investigated two redox tag placement locations on the aptamer sequence (terminal vs internal) and various sensor fabrication and interrogation parameters to tune the performance of the resulting sensor. The best-performing sensor architecture displayed a physiologically relevant dynamic range (nM) and low limit of detection and is selective among competitors and similar molecules. The development of this sensor accomplishes two breakthroughs: first, the development of a nonmicrofluidic aptamer-based electrochemical sensor that can detect NPY on a physiologically relevant (seconds to minutes) time scale and across a relevant concentration range; second, the expansion of the range of molecules for which an electrochemical, aptamer-based sensor can be used.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11214579PMC
http://dx.doi.org/10.1021/acssensors.3c00855DOI Listing

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