β-Arrestins (βarrs) are functionally versatile proteins that play critical roles in the G-protein-coupled receptor (GPCR) signaling pathways. While it is well established that the phosphorylated receptor tail plays a central role in βarr activation, emerging evidence highlights the contribution from membrane lipids. However, detailed molecular mechanisms of βarr activation by different binding partners remain elusive. In this work, we present a comprehensive study of the structural changes in critical regions of βarr1 during activation using F NMR spectroscopy. We show that phosphopeptides derived from different classes of GPCRs display different βarr1 activation abilities, whereas binding of the membrane phosphoinositide PIP stabilizes a distinct partially activated conformational state. Our results further unveil a sparsely-populated activation intermediate as well as complex cross-talks between different binding partners, implying a highly multifaceted conformational energy landscape of βarr1 that can be intricately modulated during signaling.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686989 | PMC |
| http://dx.doi.org/10.1038/s41467-023-43694-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of Longer-Term Mixed Nut Consumption on Lipoprotein Particle Concentrations in Older Adults with Overweight or Obesity.
Nutrients
December 2024
Department of Nutrition and Movement Sciences, NUTRIM Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center, 6229 ER Maastricht, The Netherlands.
Background: Recently, we reported that longer-term mixed nut intake significantly reduced serum total and low-density lipoprotein (LDL)-cholesterol, but these markers may not fully capture lipoprotein-related cardiovascular disease (CVD) risk.
Objectives: This randomized, controlled, single-blinded, crossover trial in older adults with overweight or obesity examined the effects of longer-term mixed nut consumption on lipoprotein particle size, number, and lipid distribution.
Methods: Twenty-eight participants (aged 65 ± 3 years; BMI 27.
Investigation of Active Components of (Maxim.) Franch in Mitigating Non-Alcoholic Fatty Liver Disease.
Int J Mol Sci
December 2024
Research Center for High Altitude Medicine, Key Laboratory of the Ministry of High Altitude Medicine, Key Laboratory of Applied Fundamentals of High Altitude Medicine (Qinghai-Utah Joint Key Laboratory of Plateau Medicine), Laboratory for High Altitude Medicine of Qinghai Province, Qinghai University, Xining 810001, China.
Nonalcoholic fatty liver disease (NAFLD) has rapidly emerged as the most prevalent chronic liver disease globally, representing a significant and escalating public health challenge. (Maxim.) Franch, a traditional Tibetan medicinal herb used for treating hepatitis, remains largely unexplored regarding its therapeutic potential and active components in combating NAFLD.
View Article and Find Full Text PDFShape-Persistent Tetraphenylethylene Macrocycle: Highly Efficient Synthesis and Circularly Polarized Luminescence.
Materials (Basel)
January 2025
College of Chemistry, Sichuan University, Chengdu 610064, China.
Circularly polarized luminescence (CPL) is an emerging field with significant applications in molecular electronics, optical materials, and chiroptical sensing. Achieving efficient CPL emission in organic systems remains a major challenge, particularly in the development of materials with high fluorescence quantum yields (Φ) and large luminescence dissymmetry factors (g). Herein, we report the efficient synthesis of shape-persistent tetraphenylethylene macrocycles and investigate its potential as a CPL material.
View Article and Find Full Text PDFSynthesis and Characterization of UV-Curable Resin with High Refractive Index for a Luminance-Enhancing Prism Film.
Polymers (Basel)
December 2024
Department of Chemical Engineering, Kwangwoon University, 20, Kwangwoon-ro, Nowon-gu, Seoul 01897, Republic of Korea.
A novel monomer, 9-bis[4-(2-hydroxyethoxy)phenyl]fluorene di(mercaptopropionate), with a highly refractive index, purity, and excellent UV-curable properties, is synthesized through an optimized Fischer esterification process, reacting 9,9-bis[4-(2-hydroxyethoxy)phenyl]fluorene with 3-mercaptopropionic acid. The structural characterization of this monomer is performed using Fourier-transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and liquid chromatography-mass spectrometry. The synthesis conditions are optimized using a design-of-experiments approach.
View Article and Find Full Text PDFInfluence of Parameters Used to Prepare Sterile Solutions of Poloxamer 188 on Their Physicochemical Properties.
Polymers (Basel)
December 2024
Institute of Theoretical and Experimental Biophysics RAS, Pushchino 142290, Russia.
The physicochemical properties of emulsions based on poloxamers (triblock copolymers of a hydrophobic polyoxypropylene chain and two hydrophilic polyoxyethylene chains) depend on the composition and preparation method. This study examined the impact of poloxamer P188 concentration, autoclaving mode, heating, and salt presence on the viscosity, particle size distribution, and morphology of particles using viscometric analysis, dynamic light scattering (DLS), and atomic force microscopy (AFM). It was shown that sample preparation affects the particle size and morphology but not the chemical composition of P188.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!